PMID: 9449725Mar 21, 1998Paper

Murine cytotoxic T lymphocytes recognize an epitope in an EBNA-1 fragment, but fail to lyse EBNA-1-expressing mouse cells

The Journal of Experimental Medicine
S MukherjeeA Townsend

Abstract

Major histocompatibility complex class I-restricted cytotoxic T lymphocytes (CTLs) specific for epitopes within eight of the nine Epstein Barr Virus (EBV)-encoded latency-associated proteins have been recovered from EBV-infected human subjects by restimulation of lymphocytes in vitro. However, human class I-restricted CTL responses capable of recognizing EBNA-1 expressing cells were not detected in these studies. We have raised a murine CTL line that recognizes an epitope within EBNA-1 by immunizing mice with a vaccinia virus encoding a COOH-terminal EBNA-1 fragment. This novel CTL line was used to investigate whether the epitope (positions 509-517 in EBNA-1, presented through Kd) was presented to CTL by mouse cells expressing full-length EBNA-1 or a deletion mutant of EBNA-1, lacking the Glycine-Alanine (Gly-Ala)-rich region. Cells expressing full-length EBNA-1 are not lysed by the CTL line, whereas cells expressing the Gly-Ala deletion mutant are recognized. These results suggest that epitopes from full-length EBNA-1 are poorly presented, and that the Gly-Ala-rich region is responsible for this phenomenon. The inefficient presentation of EBNA-1-derived epitopes may explain the absence or rarity of EBNA-1-specific CTLs in vivo...Continue Reading

References

Mar 1, 1991·Journal of Virology·R F AmbinderS D Hayward
Jan 1, 1989·Annual Review of Immunology·A Townsend, H Bodmer
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J DillnerR Lerner
Apr 1, 1994·Trends in Microbiology·M G Masucci, I Ernberg
Jan 1, 1993·Annual Review of Immunology·H G RammenseeO Rötzschke
Oct 9, 1996·International Journal of Cancer. Journal International Du Cancer·T FrisanM G Masucci

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Citations

Sep 26, 2002·Virus Research·Victor Levitsky, Maria G Masucci
Jan 1, 2003·Seminars in Cancer Biology·Nico P Dantuma, Maria G Masucci
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Robin Fåhraeus
May 19, 2004·The Journal of Experimental Medicine·Steven P LeeNeil W Blake
May 19, 2004·The Journal of Experimental Medicine·Judy TellamRajiv Khanna
Mar 17, 2005·PLoS Biology·Neil J BennettPhilip G Stevenson
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·N P DantumaM G Masucci
Mar 19, 2014·Nature Chemical Biology·Pierre MuratJudy Tellam
Aug 17, 1999·Current Opinion in Immunology·J I Cohen
Aug 18, 2004·The Journal of Clinical Investigation·Tihui FuRong-Fu Wang
Sep 22, 2005·Journal of Translational Medicine·Mark D McKeeMichael I Nishimura
Jun 18, 2003·Journal of Cellular Physiology·Riccardo Dolcetti, Maria G Masucci
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N BlakeA Rickinson

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