Murine embryonic EGF-responsive ventral mesencephalic neurospheres display distinct regional specification and promote survival of dopaminergic neurons

Experimental Neurology
David MosesJ Drago

Abstract

Similar to embryonic forebrain, the embryonic mesencephalon contains Fibroblast Growth Factor 2 (FGF2)- and Epidermal Growth Factor (EGF)-responsive progenitors that can be isolated as neurospheres. Developmentally, the FGF2-responsive population appears first and is thought to give rise to EGF-responsive neural stem cells. It is not known whether following this developmental switch of growth factor responsiveness ventral mesencephalic (VM)-derived neural stem cells display distinct region-specific properties. We found that murine VM- and dorsal mesencephalic (DM)-derived primary neurospheres isolated with EGF at embryonic day 14.5 differed with respect to neurosphere formation efficacy and size. VM- but not DM-derived spheres expressed En1, the molecular marker of isthmic organizer, and contained transcripts of BDNF, FGF2, IGF-I and NT-3. Both VM and DM primary neurospheres were self-renewing and gave rise to astroglial cells, but 20% of VM spheres also generated neurons. According to in vitro properties, DM- and majority of VM-derived EGF-responsive progenitors represent glial precursors. VM- but not DM-derived primary neurospheres enriched their respective conditioned medium with factors that promoted the survival of dopamin...Continue Reading

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J DragoP F Bartlett
Aug 27, 1993·Annals of the New York Academy of Sciences·D LeRoithC T Roberts
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M S RaoM Mayer-Pröschel
Apr 16, 1998·Histochemistry and Cell Biology·E DráberováP Dráber
Feb 26, 2000·Science·F H Gage
Feb 26, 2000·Science·D van der Kooy, S Weiss
Aug 10, 2000·Nature·R McKay
Apr 12, 2002·Brain Research. Developmental Brain Research·T OstenfeldC N Svendsen
Oct 16, 2002·Nature Biotechnology·Jitka OurednikEvan Y Snyder
Mar 11, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Rosanna Dono
Jun 6, 2003·The European Journal of Neuroscience·Hideki HidaHitoo Nishino
Jul 9, 2003·Annals of the New York Academy of Sciences·Mark NobleMargot Mayer-Pröschel
Sep 6, 2003·The European Journal of Neuroscience·Jesús Santa-OlallaLuis Covarrubias
Sep 23, 2003·Journal of Neurocytology·William B Stallcup
May 18, 2004·Biology of the Cell·Ying Liu, Mahendra S Rao
Aug 24, 2004·Cell and Tissue Research·Eleni Roussa, Kerstin Krieglstein
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaime ImitolaSamia J Khoury

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Citations

Apr 27, 2010·Stem Cells and Development·Chathurini V FernandoMalcolm K Horne
Jul 31, 2013·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Citlalli Regalado-SantiagoEnrique Juárez-Aguilar
Oct 27, 2007·Seminars in Cell & Developmental Biology·Vanessa J HallPatrik Brundin
Jun 23, 2007·Stem Cells·Stephanie M WillerthShelly E Sakiyama-Elbert
Jun 8, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ashok K Shetty, Bharathi Hattiangady
Dec 18, 2013·Neuroscience Letters·Shane V HegartyGerard W O'Keeffe
Nov 13, 2012·Stem Cell Research·U GalderisiM A B Melone
May 8, 2009·Biochimica Et Biophysica Acta·Ruman RahmanRichard Grundy
Jun 19, 2007·Cancer Research·Richard J Gilbertson, David H Gutmann

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