Murine embryonic stem cell-derived pancreatic acinar cells recapitulate features of early pancreatic differentiation.

Gastroenterology
Meritxell RoviraAnouchka Skoudy

Abstract

Acinar cells constitute 90% of the pancreas epithelium, are polarized, and secrete digestive enzymes. These cells play a crucial role in pancreatitis and pancreatic cancer. However, there are limited models to study normal acinar cell differentiation in vitro. The aim of this work was to generate and characterize purified populations of pancreatic acinar cells from embryonic stem (ES) cells. Reporter ES cells (Ela-pur) were generated that stably expressed both beta-galactosidase and puromycin resistance genes under the control of the elastase I promoter. Directed differentiation was achieved by incubation with conditioned media of cultured fetal pancreatic rudiments and adenoviral-mediated co-expression of p48/Ptf1a and Mist1, 2 basic helix-loop-helix transcription factors crucial for normal pancreatic acinar development and differentiation. Selected cells expressed multiple markers of acinar cells, including digestive enzymes and proteins of the secretory pathway, indicating activation of a coordinated differentiation program. The genes coding for digestive enzymes were not regulated as a single module, thus recapitulating what occurs during in vivo pancreatic development. The generated cells displayed transient agonist-induce...Continue Reading

Citations

Jun 10, 2011·Molecular & Cellular Proteomics : MCP·Shalini MakawitaEleftherios P Diamandis
Oct 5, 2010·Stem Cells and Development·Sakiko ShirasawaKatsunori Sasaki
Feb 6, 2017·Gut·Victor J Sánchez-Arévalo LoboFrancisco X Real
Sep 3, 2013·Cell and Tissue Research·Sakiko Takizawa-ShirasawaKatsunori Sasaki
Sep 14, 2012·Journal of Molecular Histology·JiaQing GongYonghua Wang
Nov 13, 2019·Journal of Morphology·Magdalena Kowalska, Weronika Rupik
Dec 3, 2020·International Journal of Molecular Sciences·Mahmoud MonaSeunghee Cha
Apr 19, 2012·Gastroenterology·Daniel DirenzoStephen F Konieczny
Jun 21, 2011·Biochemical and Biophysical Research Communications·Sakiko ShirasawaKatsunori Sasaki
Dec 8, 2021·Cellular and Molecular Gastroenterology and Hepatology·Elyne BackxIlse Rooman

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