Murine in vitro whole bladder model: a method for assessing phenotypic responses to pharmacologic stimuli and hypoxia

Neurourology and Urodynamics
Joel C HutchesonStephen A Zderic

Abstract

Recent advances in genetic manipulation have allowed for over expression or deletion of selective genes in mice. This offers urologic investigators new means of understanding bladder function in the context of normal development or the response to outlet obstruction. It is important to correlate any genetic manipulations in mice with specific phenotypic properties such as voiding patterns, or muscle strip physiology. We describe a simple in vivo whole bladder preparation that may be used to study the phenotypic changes in bladder function. Murine bladders were mounted on a 30 gauge needle and mounted in an organ chamber containing a physiologic buffer solution. Passive bladder properties were assessed with cystometry, and active contractile responses were measured in response to electrical field stimulation and agonists. The effects of hypoxia were also studied. Compliance in the murine bladder is dependent upon actin myosin interactions, and increased in the presence of calcium free buffer and EGTA. The sarcoplasmic reticulum plays a smaller role in the contraction of murine bladder than in other species. Murine bladder smooth muscle demonstrated a remarkable ability to withstand hypoxia. This simple model can be adapted to he...Continue Reading

References

Nov 1, 1982·The Journal of Urology·R M Levin, A J Wein
Jan 1, 1995·Advances in Experimental Medicine and Biology·S A ZdericR M Levin
Jan 1, 1995·Advances in Experimental Medicine and Biology·C A Peters
Jan 1, 1995·Advances in Experimental Medicine and Biology·D E CoplenE J Macarak
Mar 1, 1997·The Journal of Urology·R S SutherlandD S Bredt
Jan 1, 1997·Neurourology and Urodynamics·D RohrmannM S Damaser
Aug 1, 1997·The Journal of Urology·L T O'ConnorD Felsen
Sep 1, 1997·The Journal of Urology·D RohrmannS A Zderic
Jan 14, 1998·Urology·A F Brading
Aug 24, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K PerssonK E Andersson

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Citations

Mar 4, 2011·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Joseph Thomas
Mar 11, 2011·Revista da Associação Médica Brasileira·Gisela Rosa FrancoRodrigo Aquino Castro

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