May 10, 2018

Murine intestinal organoids resemble intestinal epithelium in their microRNA profiles

Bioscience, Biotechnology, and Biochemistry
Fumina Ohsaka, Kei Sonoyama

Abstract

Intestinal organoids were established as an ex vivo model of the intestinal epithelium. We investigated whether organoids resemble the intestinal epithelium in their microRNA (miRNA) profiles. Total RNA samples were obtained from crypt and villus fractions in murine intestine and from cultured organoids. Microarray analysis showed that organoids largely resembled intestinal epithelial cells in their miRNA profiles. In silico prediction followed by qRT-PCR suggested that six genes are regulated by corresponding miRNAs along the crypt-villus axis, suggesting miRNA regulation of epithelial cell renewal in the intestine. However, such expression patterns of miRNAs and their target mRNAs were not reproduced during organoids maturation. This might be due to lack of luminal factors and endocrine, nervous, and immune systems in organoids and different cell populations between in vivo epithelium and organoids. Nevertheless, we propose that intestinal organoids provide a useful in vitro model to investigate miRNA expression in intestinal epithelial cells.

  • References22
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Citations

Mentioned in this Paper

In Vivo
Immune System
Microarray Analysis
Intestinal Villus
Patterns
MicroRNA Gene
Genes
Regulation of Biological Process
Squamous Transitional Epithelial Cell Count
Murine

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