Murine Ksr interacts with MEK and inhibits Ras-induced transformation

Current Biology : CB
A Denouel-GalyA Eychène

Abstract

Ksr (kinase supressor of Ras) was identified as a regulator of the Ras-MAP kinase (mitogen-activated protein kinase) pathway by genetic screens in Drosophila and Caenorhabditis elegans. Ksr is a kinase with similarities to the three conserved regions of Raf kinases, especially within the kinase domain. To investigate whether these structural similarities correlated with common functional properties, we examined the ability of mKsr-1, the murine homolog of Ksr, to interact with components of the vertebrate MAP kinase pathway. In the yeast two-hybrid interaction assay, mKsr-1 did not bind to either Ras, B-Raf or Raf-1, but interacted strongly with both MEK-1 and MEK-2, activators of MAP kinase. The Ksr-MEK interaction was confirmed by co-immunoprecipitation experiments. Ectopically expressed mKsr-1 co-precipitated with endogenous MEK-1 in COS-1 cells, and endogenous Ksr and MEK co-precipitated from PC12 cells. Phosphorylation of MEK by mKsr-1 was not detected, however. In contrast, the MEK subpopulation complexed with mKsr-1 in COS-1 cells or PC12 cells did not display kinase activity. This ability of Ksr to block MEK in an inactive form correlated with a biological response: mKsr-1 did not transform NIH3T3 cells, and, furthermor...Continue Reading

References

Nov 1, 1995·Molecular and Cellular Biology·R Khosravi-FarC J Der
Mar 30, 1995·Nature·R G QiuM Symons
Apr 28, 1995·The Journal of Biological Chemistry·T R BrtvaC J Der
Nov 1, 1994·Molecular and Cellular Biology·A KikuchiL T Williams
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·F HoferG S Martin
Aug 18, 1994·Nature·P Rodriguez-VicianaJ Downward
Apr 25, 1994·Nucleic Acids Research·H E FeilotterD Beach
Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J WuT W Sturgill
Apr 1, 1993·Molecular and Cellular Biology·J PosadaJ A Cooper
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G QiuM Symons
Dec 1, 1995·Current Opinion in Cell Biology·A J Waskiewicz, J A Cooper
Jul 12, 1996·The Journal of Biological Chemistry·M A WhiteM H Wigler
Nov 1, 1996·Genes & Development·M TherrienD K Morrison
Jan 10, 1997·The Journal of Biological Chemistry·J M BrondelloF R McKenzie
May 1, 1997·Current Biology : CB·H XingA J Muslin
Jul 24, 1997·Oncogene·E Douville, J Downward

❮ Previous
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Citations

May 5, 2001·Pharmacology & Therapeutics·C R Weinstein-OppenheimerJ A McCubrey
Dec 4, 2001·Biology of the Cell·C Peyssonnaux, A Eychène
Jun 5, 2003·Biochimica Et Biophysica Acta·Kathryn E Mercer, Catrin A Pritchard
Mar 14, 2000·Current Opinion in Cell Biology·W R Burack, A S Shaw
Feb 4, 2012·Cell Death and Differentiation·M W Gramling, C M Eischen
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Claudia WellbrockRichard Marais
May 15, 2007·Oncogene·M M McKay, D K Morrison
May 15, 2007·Oncogene·A Clapéron, M Therrien
May 15, 2007·Oncogene·D N DhanasekaranE P Reddy
Sep 8, 2010·Médecine sciences : M/S·Antoine Galmiche, Zakaria Ezzoukhry
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Melissa M McKayDeborah K Morrison
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·M TherrienG M Rubin
Apr 11, 2001·The EMBO Journal·M HüserC Pritchard
Feb 19, 2002·Genes & Development·François RoyMarc Therrien
Nov 1, 2006·Molecular and Cellular Biology·Isabelle HmitouAlain Eychène
Jul 18, 2012·Molecular and Cellular Biology·Mario R FernandezRobert E Lewis
Sep 13, 2000·Molecular and Cellular Biology·C PeyssonnauxA Eychène
Sep 5, 2001·Molecular and Cellular Biology·O ZugastiU Hibner
May 4, 2004·Molecular and Cellular Biology·Robert L Kortum, Robert E Lewis
Jun 1, 2005·Molecular and Cellular Biology·Ashok PullikuthAndrew D Catling
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Deborah K Morrison, Roger J Davis
Nov 14, 2007·BMC Cell Biology·Sarah J AnnesleyPaul R Fisher
Jun 28, 2013·PloS One·Myoungkun JeoungEmilia Galperin
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tomas VomastekMichael J Weber
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hema VaidyanathanJoe W Ramos
Nov 30, 2005·Molecular Biology of the Cell·Sarah E RobertsonMargaret M Chou
Feb 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Natalia V KirienkoDavid S Fay
Nov 18, 2004·Journal of Cellular Biochemistry·Charu SharmaMichael J Weber
Jul 19, 2013·Biochemical Society Transactions·Hua ZhangGeorgios Giamas
Dec 2, 1999·Experimental Cell Research·C Hagemann, U R Rapp
Dec 2, 1999·Experimental Cell Research·J EnglishM H Cobb
Mar 21, 2000·Seminars in Immunology·M F Olson, R Marais
Nov 3, 2004·The Journal of Clinical Investigation·Richard Kolesnick, H Rosie Xing
Jun 30, 2009·Molecular Cell·Michele K DoughertyDeborah K Morrison
Mar 23, 2002·Biochimica Et Biophysica Acta·Xiang L YinJian X Gu
Feb 6, 2007·Biochimica Et Biophysica Acta·John M Kyriakis

❮ Previous
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