Murine mechanical ventilation stimulates alveolar epithelial cell proliferation.

Experimental Lung Research
Patricia Rose ChessCarl J Johnston

Abstract

High tidal volume mechanical ventilation can cause inflammation and lung damage. Mechanical strain is also necessary for normal lung growth. The current work was performed to determine if mechanical ventilation with clinically utilized tidal volumes stimulates a proliferative response in the lung. Six- to 8-week-old C57/Bl6 mice, anesthetized with ketamine/xylozine, were ventilated for 6 hours with 10 mL/kg tidal volume, positive end-expiratory pressure (PEEP) 3cm H(2)O. Pulmonary function testing demonstrated decreased compliance within 3 hours of ventilation. Assessment of bronchoalveolar lavage (BAL) demonstrated no significant increase in lactate dehydrogenase, total lavagable cell number, or total protein after ventilation. There was evidence of inflammation in the lungs of ventilated mice, with an increased percentage of lymphocytes and neutrophils in BAL, and an increase in macrophage inflammatory protein (MIP)-2 and interleukin (IL)-1beta message in lung tissue. Immunohistochemistry of inflation-fixed lungs demonstrated increased alveolar cell proliferation, as measured by both proliferating cell nuclear antigen and Ki67 staining. Dual staining confirmed that proliferating cells labeled with proSP-B, demonstrating that ...Continue Reading

References

Oct 1, 1975·The Journal of Clinical Investigation·J S Brody
Mar 1, 1991·Critical Care Medicine·W K AdkinsJ C Parker
Sep 1, 1990·Journal of Applied Physiology·K TsunoT Kolobow
Jan 1, 1988·Pediatric Pulmonology·P T CagleW M Thurlbeck
May 1, 1989·Journal of Applied Physiology·L A HernandezJ C Parker
Sep 1, 1993·Experimental Lung Research·H S SekhonW M Thurlbeck
Oct 1, 1996·Journal of Applied Physiology·S ZhangJ T McBride
Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyJ N Finkelstein
May 4, 2000·The New England Journal of Medicine·UNKNOWN Acute Respiratory Distress Syndrome NetworkArthur Wheeler
Aug 3, 2000·Intensive Care Medicine·C C dos Santos, A S Slutsky
Mar 10, 2001·AACN Clinical Issues·M H van SoerenW M Haddara
Nov 5, 2002·The European Respiratory Journal·U UhligS Uhlig
May 20, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ozlem U GurkanPatrice M Becker
Dec 3, 2003·American Journal of Respiratory and Critical Care Medicine·Li-Fu LiDeborah A Quinn
Jan 24, 2004·The European Respiratory Journal·M MayC P Speer
Oct 19, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael R WilsonMasao Takata
Nov 13, 2004·Annals of Biomedical Engineering·Lennart K A LundbladJason H T Bates
Dec 25, 2004·Seminars in Thoracic and Cardiovascular Surgery·Daniel KreiselCharles B Huddleston
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Nicholas E Vlahakis, Rolf D Hubmayr
May 24, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shwu-Fan MaJoe G N Garcia
Nov 22, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jooeun LeeBarbara Driscoll
Jul 21, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shireesha DhanireddyW Conrad Liles
Aug 29, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daisuke OkutaniMingyao Liu
Jun 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alessandra PaganoConstance Barazzone Argiroffo
Jun 30, 2007·European Journal of Pharmacology·Akihiro SakashitaMitsuhiro Yokoyama
Jul 3, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leena P DesaiChristopher M Waters
Jul 14, 2007·American Journal of Respiratory and Critical Care Medicine·Scott E SinclairChristopher M Waters
Sep 18, 2007·Journal of Applied Physiology·Stephen J Lai-FookYih-Loong Lai
Nov 3, 2007·The Journal of Thoracic and Cardiovascular Surgery·Lucas G FernandezVictor E Laubach
Feb 23, 2008·Anesthesia and Analgesia·Athanasios G KaditisJoseph Milic-Emili
May 5, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Richard L AutenMulugu Brahmajothi
Jun 23, 2009·Respiratory Physiology & Neurobiology·Dani-Louise DixonAndrew D Bersten
Sep 1, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Megan O'ReillyFoula Sozo

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Citations

Mar 29, 2014·Critical Care : the Official Journal of the Critical Care Forum·Jan HirschJames A Frank
Mar 21, 2020·Frontiers in Endocrinology·Beth J AllisonSuzanne L Miller
Apr 6, 2018·Oxidative Medicine and Cellular Longevity·Ana Beatriz Farias de SouzaFrank Silva Bezerra
Nov 18, 2020·Respiratory Physiology & Neurobiology·Matheus Rocha AlmeidaFrank Silva Bezerra
Oct 31, 2021·Laboratory animal research·Jon Petur JoelssonSigurbergur Karason

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