Aug 1, 1989

Murine monoclonal antibodies which recognize active sites of Escherichia coli NusA protein and epitope mapping by gene fusion

Gene
Y NakamuraD L Court

Abstract

Seven hybridomas producing murine monoclonal antibodies reactive against NusA protein of Escherichia coli were prepared. Antigenic determinants of these monoclonal antibodies have been mapped by immunoblotting analyses using fusion proteins containing parts of NusA. The epitope of the N14 antibody maps in a hydrophobic amino acid (aa) cluster and consists of at least Ala-181 and Ser-183 residues. nusA1 and nusA11 mutations, which cause aa changes of these residues, abolish the antigenic reactivity to the N14 antibody. These antibodies react with intact NusA protein, indicating that the epitopes are exposed on the surface of NusA. Most of these epitopes cluster around the nusA1 and nusA11 mutation loci.

  • References19
  • Citations1

Citations

Mentioned in this Paper

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Bacterial Proteins
Alkalescens-Dispar Group
Mice, Inbred BALB C
Binding Sites, Antibody
Murine
Antigenic Specificity
Immunoblotting
Gene Fusion Abnormality

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