Murine Polyomavirus Cell Surface Receptors Activate Distinct Signaling Pathways Required for Infection

Samantha D O'Hara, Robert L Garcea


Virus binding to the cell surface triggers an array of host responses, including activation of specific signaling pathways that facilitate steps in virus entry. Using mouse polyomavirus (MuPyV), we identified host signaling pathways activated upon virus binding to mouse embryonic fibroblasts (MEFs). Pathways activated by MuPyV included the phosphatidylinositol 3-kinase (PI3K), FAK/SRC, and mitogen-activated protein kinase (MAPK) pathways. Gangliosides and α4-integrin are required receptors for MuPyV infection. MuPyV binding to both gangliosides and the α4-integrin receptors was required for activation of the PI3K pathway; however, either receptor interaction alone was sufficient for activation of the MAPK pathway. Using small-molecule inhibitors, we confirmed that the PI3K and FAK/SRC pathways were required for MuPyV infection, while the MAPK pathway was dispensable. Mechanistically, the PI3K pathway was required for MuPyV endocytosis, while the FAK/SRC pathway enabled trafficking of MuPyV along microtubules. Thus, MuPyV interactions with specific cell surface receptors facilitate activation of signaling pathways required for virus entry and trafficking. Understanding how different viruses manipulate cell signaling pathways thr...Continue Reading


Jan 13, 2018·Journal of Virology·Jeanne K DuShaneMelissa S Maginnis


Aug 1, 1975·Virology·E Goldman, Thomas Benjamin
May 1, 1990·Journal of Virology·G M Glenn, W Eckhart
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J ZulloRobert L Garcea
Jan 1, 1995·Journal of Neuro-oncology·A J YatesJ Van Brocklyn
Jun 22, 2000·The Journal of Biological Chemistry·R LiStephan Ladisch
May 23, 2002·Cellular and Molecular Life Sciences : CMLS·U F Greber
Jan 29, 2003·Journal of Virology·Joanna M GilbertThomas Benjamin
Mar 14, 2003·Journal of Virology·Maddalena CarusoMarie-Isabelle Garcia
Aug 28, 2003·The EMBO Journal·Billy TsaiTom A Rapoport
Jun 25, 2004·The Journal of Biological Chemistry·Yihui LiuStephan Ladisch
May 17, 2005·Nature Cell Biology·Ellen J EzrattyGregg G Gundersen
Sep 15, 2005·Journal of Cell Science·Abdelkader HamadiPhilippe Rondé
Jan 18, 2006·Journal of Virology·Jonathan A LowMichael J Imperiale
Nov 30, 2006·Methods in Enzymology·Karen KaucicStephan Ladisch
Jan 30, 2008·Current Protocols in Cell Biology·Christopher B Buck, Cynthia D Thompson
Sep 5, 2008·PLoS Pathogens·Mohammad F SaeedRobert A Davey
Nov 11, 2008·Virology·Mengxi JiangMichael J Imperiale
Feb 26, 2009·Genes & Development·Kyle R LegateReinhard Fässler
Jul 17, 2009·Journal of Virology·Kimberly D EricksonBilly Tsai
Oct 31, 2009·Cellular Signalling·Annette M GonzalezJonathan C R Jones
Jan 22, 2010·Journal of Virology·Veronika Butin-IsraeliAriella Oppenheim
Feb 26, 2010·Journal of Virology·Alyson I SwimmDaniel Kalman
Jul 30, 2010·Journal of Virology·Mengding Qian, Billy Tsai
Oct 26, 2012·The Journal of Biological Chemistry·Zhe LiuJing-hsiung James Ou
Dec 20, 2013·Viruses·Nora Diehl, Heiner Schaal

Related Concepts

Tumor Virus Infections
Src-Family Kinases
JUN gene
Immune Response
Flow Cytometry
Immunofluorescence Assay

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.