Jun 1, 1989

Murine tissue macrophages synthesize and secrete amyloid proteins different to amyloid A (AA)

European Journal of Clinical Investigation
G RamadoriK H Meyer zum Büschenfelde


We recently demonstrated that serum amyloid A (SAA) gene expression can be induced in extrahepatic sites with exception of the brain. Furthermore we demonstrated that tissue macrophages express SAA-gene constitutively and that SAA-gene expression can be increased by endotoxin. Until now the protein corresponding to the SAA-specific mRNA contained in macrophages has not been identified. We compared proteins precipitated from endogenously labelled samples by three antisera against amyloid A (AA) raised in different laboratories. Radiolabelled samples were derived from murine hepatocyte cultures, from cell-free translation of acute phase liver RNA or hepatocytes RNA and from peritoneal macrophages as well as Kupffer cells. All three antisera recognize a protein of 12.5 kDa Mr produced by hepatocytes (SAA), and a major protein of 14.3 kDa Mr contained in the cell-free translation products; this is the precursor of the mature SAA as demonstrated by cleavage experiments with canine pancreas microsomal enzymes. The antisera also recognize two proteins--a major one of 14.5 kDa Mr and a second of 12.5 kDa Mr contained in the supernatants and cell lysates of liver and peritoneal macrophages. New antisera raised against the two proteins d...Continue Reading

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Mentioned in this Paper

Hepatic Macrophage
Macrophages, Peritoneal
Precipitin Tests
APP protein, human
Benign Tumor of Pancreas
Immune Sera
Cytokinesis of the Fertilized Ovum

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