Murine Wnt-1 with an internal c-myc tag recombinantly produced in Escherichia coli can induce intracellular signaling of the canonical Wnt pathway in eukaryotic cells.

The Journal of Biological Chemistry
Beatrix FahnertPeter Neubauer

Abstract

Wnt-1 belongs to the Wnt family of secreted glycoproteins inducing an intracellular signaling pathway involved in cell proliferation, differentiation, and pattern formation. The canonical branch is one of three known branches. This is also valid in vitro, and Wnts can be considered beneficial for culturing primary cells from organs, provided Wnts are available and applicable even with cells of different species. It was shown here that internally c-myc-tagged murine Wnt-1 produced in the heterologous host Escherichia coli was appropriate for inducing intracellular signaling of the canonical Wnt pathway in eukaryotic cells via stabilization of cytosolic beta-catenin. The pioneering injection of the protein into the blastocoels of Xenopus laevis embryos led to axis duplication and suppression of head formation. Applying the recombinant murine Wnt-1 to metanephric mesenchyme activated the tubulogenic program. The signal-inducing activity of the recombinant protein was also positively demonstrated in the TOP-flash reporter assay. Although Wnts were purified recently from the growth media of stably transfected eukaryotic cell lines, the production of active Wnt proteins in pro- or eukaryotic microorganisms reportedly has never been s...Continue Reading

References

Aug 1, 1995·Molecular and Cellular Biology·R S Bradley, A M Brown
Aug 1, 1993·Current Opinion in Biotechnology·C H Schein
Jan 1, 1995·Developmental Biology·J A Davies, D R Garrod
May 1, 1997·Mechanisms of Development·Y Landesman, S Y Sokol
Dec 3, 1998·Applied and Environmental Microbiology·J QiuG Georgiou
Aug 4, 1999·Trends in Genetics : TIG·C Niehrs
Mar 8, 2000·Mechanisms of Development·S KuureS Vainio
Feb 17, 2001·The EMBO Journal·C Niehrs
Sep 13, 2001·Current Biology : CB·T P Yamaguchi
Feb 9, 2002·Molecular Reproduction and Development·Johannes Müllegger, Günter Lepperdinger
Jul 26, 2002·Seminars in Cell & Developmental Biology·Michael Kühl
Jul 26, 2002·The Journal of Biological Chemistry·Takatoshi KarasawaPaul J Lombroso
Oct 3, 2002·Journal of Cell Science·Joerg Huelsken, Juergen Behrens
Nov 7, 2002·Current Biology : CB·Carole LaBonne
Apr 30, 2003·Nature·Tannishtha ReyaIrving L Weissman

❮ Previous
Next ❯

Citations

Oct 22, 2005·Infection and Immunity·Miguel A AscónDavid W Pascual
Apr 26, 2005·Microbial Cell Factories·Salvador Ventura
Jul 28, 2009·Microbial Cell Factories·Germán L Rosano, Eduardo A Ceccarelli
Mar 24, 2005·Journal of Molecular Biology·Mar CarrióSalvador Ventura
Jun 23, 2007·Biochemical and Biophysical Research Communications·Riffat NaseemAnthony K Campbell
Dec 6, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Joshua N FarrDavid G Monroe

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.