Mus musculus-microRNA-449a ameliorates neuropathic pain by decreasing the level of KCNMA1 and TRPA1, and increasing the level of TPTE

Molecular Medicine Reports
Shan LuGuoqing Zhao

Abstract

Neuropathic pain is a nerve disorder characterized by the dysregulation of ion channels in dorsal root ganglion (DRG) neurons. MicroRNAs (miRs) may be associated with the molecular mechanisms underlying the altered levels of ion channels; however, the molecular mechanisms remain widely unknown. To investigate these mechanisms, the present study conducted a genomic analysis of miR between a unilateral spared nerve injury (SNI) model and sham control. Differentially expressed miRs between the SNI and sham groups were selected for transfection of DRG cells, a polymerase chain reaction (PCR) array analysis was subsequently performed. A total of three significantly differently expressed genes were selected from the results of the PCR array and further analyzed by reverse transcription‑quantitative PCR. Genomic analysis revealed that Mus musculus miR‑449a (mmu‑miR‑449a) was reduced in the SNI groups compared with the sham controls. The PCR array indicated that mmu‑miR‑449a‑transfection reduced the mRNA expression levels of transient receptor potential cation channel subfamily A member 1 (TRPA1), and calcium‑activated potassium channel subunit α‑1 (KCNMA1) and increased the level of transmembrane phosphatase with tension homology (TPT...Continue Reading

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Citations

Sep 14, 2017·International Journal of Molecular Sciences·Noopur BhoreYung-Feng Liao
May 10, 2020·Anesthesia and Analgesia·Juan P CataHolger K Eltzschig
Sep 18, 2020·Expert Opinion on Investigational Drugs·Asia Fernández-CarvajalAntonio Ferrer-Montiel
Nov 9, 2017·Journal of Cellular Physiology·Ying ZhaoChang-Ming Zhao
Jun 15, 2019·International Journal of Molecular Sciences·Simona GiorgiSara González-Rodríguez
Jun 27, 2020·Neuronal Signaling·Theodora KalpachidouMichaela Kress
Jul 8, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ge SongXueqiang Wang

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