Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep

Cell Reports
Yasutaka NiwaHiroki R Ueda

Abstract

Sleep regulation involves interdependent signaling among specialized neurons in distributed brain regions. Although acetylcholine promotes wakefulness and rapid eye movement (REM) sleep, it is unclear whether the cholinergic pathway is essential (i.e., absolutely required) for REM sleep because of redundancy from neural circuits to molecules. First, we demonstrate that synaptic inhibition of TrkA+ cholinergic neurons causes a severe short-sleep phenotype and that sleep reduction is mostly attributable to a shortened sleep duration in the dark phase. Subsequent comprehensive knockout of acetylcholine receptor genes by the triple-target CRISPR method reveals that a similar short-sleep phenotype appears in the knockout of two Gq-type acetylcholine receptors Chrm1 and Chrm3. Strikingly, Chrm1 and Chrm3 double knockout chronically diminishes REM sleep to an almost undetectable level. These results suggest that muscarinic acetylcholine receptors, Chrm1 and Chrm3, are essential for REM sleep.

References

Sep 11, 1990·Nucleic Acids Research·S Mizushima, S Nagata
Jun 14, 1968·Science·D J McGinty, M B Sterman
Jul 14, 1983·Nature·F Crick, G Mitchison
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·W XuA L Beaudet
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anthony S BasileJurgen Wess
Nov 1, 1957·Electroencephalography and Clinical Neurophysiology·W DEMENT, N KLEITMAN
Dec 1, 1964·International Journal of Neuropharmacology·R GEORGED J JENDEN
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Jürgen Wess
Jan 15, 2005·Progress in Neurobiology·C Gotti, F Clementi
Feb 26, 2005·Nucleic Acids Research·Søren WarmingNeal G Copeland
Aug 20, 2005·Neuropsychobiology·Romain GoutagnyPatrice Fort
May 3, 2006·Genesis : the Journal of Genetics and Development·Itaru ImayoshiRyoichiro Kageyama
May 12, 2006·Nature·Jun LuClifford B Saper
Dec 8, 2006·Nature·Ed S LeinAllan R Jones
Sep 1, 2007·Science·Leon G ReijmersMark Mayford
Oct 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Norio WadaShigetada Nakanishi
Apr 7, 2009·Bioinformatics·Stephan PreibischPavel Tomancak
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ariel KamslerSusumu Tonegawa
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hiromasa FunatoMasashi Yanagisawa
Jul 1, 1946·Journal of Neurophysiology·W J H NAUTA
Aug 16, 2011·Brain Research·Oscar V RamosJack Yamuy
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 20, 2012·Physiological Reviews·Ritchie E BrownRobert W McCarley
Jan 5, 2013·Science·Le CongFeng Zhang
Jan 31, 2013·Nature Biotechnology·Seung Woo ChoJin-Soo Kim
Apr 30, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Genshiro A SunagawaHiroki R Ueda
Jun 14, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kaori TsujinoHiroki R Ueda
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Dec 18, 2013·The Journal of Physiology·F J WengE Arrigoni
Oct 9, 2015·Nature Protocols·Etsuo A SusakiHiroki R Ueda

❮ Previous
Next ❯

Citations

Jun 19, 2019·The European Journal of Neuroscience·Kazuya Sakai
Aug 30, 2019·PLoS Computational Biology·Jacob Bradford, Dimitri Perrin
Feb 6, 2020·Frontiers in Neuroscience·Rikuhiro G Yamada, Hiroki R Ueda
Apr 11, 2020·F1000Research·Maxime JanPaul Franken
Aug 15, 2020·Frontiers in Psychology·Sung-Ho Park, Franz Weber
Nov 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Phillip A ClevesJohn R Pringle
Nov 25, 2020·Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine·Milan NigamIsabelle Arnulf
Jan 26, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Tian TianAiguo Ren
Aug 24, 2020·Neuroscience·Marco FuenzalidaAndrés E Chávez
Apr 8, 2019·Current Opinion in Biotechnology·Hideki UkaiHiroki R Ueda
Apr 2, 2021·Frontiers in Neural Circuits·Susheel Vijayraghavan, Stefan Everling
Apr 27, 2021·Frontiers in Neuroscience·Yuri Panchin, Vladimir M Kovalzon
May 13, 2021·Journal of Radiation Research·Kento MiuraOsamu Kaminuma
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shinhui LeeJunho H Lee
Jul 3, 2021·Clocks & Sleep·Maria Neus Ballester RoigValérie Mongrain
Dec 29, 2020·Current Opinion in Neurobiology·John M Webb, Ying-Hui Fu
Oct 29, 2021·Science·Nicholas P Franks, William Wisden

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Christa J Van Dort, Emery N Brown
American Journal of Ophthalmology
A KalesJ T Pearlman
Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology
A Imagawa, S Akazawa
© 2022 Meta ULC. All rights reserved