Muscarinic acetylcholine receptors in the brain of the zebrafish (Danio rerio) measured by radioligand binding techniques

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Frederick E Williams, William S. Messer

Abstract

Muscarinic acetylcholine receptors (mAChRs) play a role in learning, memory and behavior in vertebrate animals. We measured the muscarinic cholinergic receptor levels in extracts from zebrafish (Danio rerio) brain by radioligand binding techniques. Saturation binding experiments with the radioligand [3H]-quinuclidinyl benzilate (QNB) were used to determine receptor number and relative affinity for several agonists and antagonists. Affinity at zebrafish brain receptors was relatively high with a K(d) of 40 +/- 5 pM. The number of receptors, represented by Bmax, was 63 +/- 16 fmol/mg protein. Oxotremorine and carbachol, agonists at muscarinic acetylcholine receptors, bound with displacement curves indicating multiple binding sites. In addition, oxotremorine bound with a higher affinity than did carbachol. The antagonist potency profile at zebrafish receptors in brain was determined to be atropine>pirenzipine>p-fluoro-hexahydro-sila-difenidol>otenzepad. The results obtained with zebrafish brain compare favorably to those found in insect, fish and mammalian species. Taken together, the binding results and favorable comparisons to mammalian systems indicate that zebrafish may provide a useful model organism for evaluating the role o...Continue Reading

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Citations

Jun 22, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jonathan D BestAlan G Roach
Jun 17, 2010·The Journal of Biological Chemistry·Lung-Kun YehI-Jong Wang
May 15, 2008·Pigment Cell & Melanoma Research·Kenji Hatamoto, Chikako Shingyoji
Sep 13, 2011·Neurotoxicology and Teratology·E P RicoC D Bonan
May 27, 2008·Neuroscience Letters·Eunjin ParkChang-Joong Lee
Aug 23, 2012·The Journal of Comparative Neurology·Brenda Toscano-MárquezRüdiger Krahe
May 14, 2011·Developmental Neurobiology·Jason Rihel, Alexander F Schier
Jun 30, 2006·Neurotoxicology and Teratology·Eduardo Pacheco RicoCarla Denise Bonan
Oct 27, 2020·Frontiers in Psychiatry·Veronika LangovaJiri Horacek
Mar 10, 2021·Neurotoxicity Research·Baban S Thawkar, Ginpreet Kaur

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