Muscarinic activation of a novel voltage-sensitive inward current in rabbit prevertebral sympathetic neurons

The European Journal of Neuroscience
P DelmasM Gola

Abstract

The muscarinic activation of rabbit prevertebral sympathetic neurons was studied in non-dissociated coeliac and superior mesenteric ganglia using whole-cell patch-clamp techniques. In the presence of nicotinic blockers, carbachol, muscarine and oxotremorine-M (1-50 microM) induced tonic firing by activating a persistent inward current. These effects were abolished by atropine. They persisted when the M-current was blocked with Ba2+ (1 mM) and intracellular Cs+. The muscarinic inward current was found to be time- and voltage-dependent. It peaked at -60 mV, decreased at large hyperpolarizations and was tonically activated between -110 and -20 mV, which gave steady-state I-V curves an N-shape between -96 and -54 mV. The negative slope accounted for the large hyperpolarizing responses generated by current pulses in carbachol-treated cells. The muscarinic current was abolished when Na+ was replaced by choline, Tris+, sucrose, N-methyl-D-glucamine and Cs+ but not Li+. It was resistant to tetrodotoxin (3 microM), amiloride (3 microM), benzamil (10 microM) and tetraethylammonium (5-20 mM). No involvement of K+ and Cl- could be detected. We therefore styled it INa,M, in reference to its ionic selectivity and its coupling to muscarinic r...Continue Reading

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Citations

Dec 24, 1997·Brain Research. Brain Research Reviews·P DelmasM Gola
Jan 8, 2011·Nature Protocols·Nancy OsorioPeter A Jones
Oct 12, 2001·The European Journal of Neuroscience·P PiersonM Raggenbass
May 17, 2002·The Journal of Physiology·Shih-Chieh ChuangRobert K S Wong
Jul 8, 2009·Autonomic Neuroscience : Basic & Clinical·Caroline Fasano, Jean-Pierre Niel
Feb 21, 1998·Journal of Neurophysiology·G Svirskis, J Hounsgaard

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