Muscarinic and nicotinic ACh receptor activation differentially mobilize Ca2+ in rat intracardiac ganglion neurons

Journal of Neurophysiology
Friederike BekerD J Adams

Abstract

The origin of intracellular Ca2+ concentration ([Ca2+]i) transients stimulated by nicotinic (nAChR) and muscarinic (mAChR) receptor activation was investigated in fura-2-loaded neonatal rat intracardiac neurons. ACh evoked [Ca2+]i increases that were reduced to approximately 60% of control in the presence of either atropine (1 microM) or mecamylamine (3 microM) and to <20% in the presence of both antagonists. Removal of external Ca2+ reduced ACh-induced responses to 58% of control, which was unchanged in the presence of mecamylamine but reduced to 5% of control by atropine. The nAChR-induced [Ca2+]i response was reduced to 50% by 10 microM ryanodine, whereas the mAChR-induced response was unaffected by ryanodine, suggesting that Ca2+ release from ryanodine-sensitive Ca2+ stores may only contribute to the nAChR-induced [Ca2+]i responses. Perforated-patch whole cell recording at -60 mV shows that the rise in [Ca2+]i is concomitant with slow outward currents on mAChR activation and with rapid inward currents after nAChR activation. In conclusion, different signaling pathways mediate the rise in [Ca2+]i and membrane currents evoked by ACh binding to nicotinic and muscarinic receptors in rat intracardiac neurons.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L BernheimB Hille
Aug 1, 1992·Journal of the Autonomic Nervous System·A A Selyanko, V I Skok
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Jul 1, 1995·The Journal of Physiology·S X Xi-Moy, N J Dun
May 1, 1994·Trends in Pharmacological Sciences·B E EhrlichI Bezprozvanny
Jul 1, 1994·Physiological Reviews·T PozzanJ Meldolesi
Jan 1, 1996·Cell Calcium·A Verkhratsky, A Shmigol
Apr 1, 1996·The American Journal of Physiology·B C KimballM W Mulholland
Jan 15, 1997·The Journal of Physiology·L L DassoR D Vaughan-Jones
Dec 24, 1997·Journal of the Autonomic Nervous System·M F KlemmG D Hirst
Aug 11, 1998·Neuron·M J Berridge
Jul 2, 1999·Biophysical Journal·M MuscholB M Salzberg
Jul 22, 1999·Annals of the New York Academy of Sciences·D S McGehee
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Sharma, S Vijayaraghavan
May 13, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sukwoo ChoiRichard W Tsien

❮ Previous
Next ❯

Citations

May 7, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jhansi DyavanapalliAlexander A Harper
Aug 26, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Allison E GirasoleJean C Hardwick
May 23, 2014·The Journal of Steroid Biochemistry and Molecular Biology·M HillL Stárka
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
May 29, 2004·Trends in Pharmacological Sciences·Federico Dajas-Bailador, Susan Wonnacott
May 6, 2015·Neuropharmacology·Michiko HirayamaHitoshi Ishibashi
Sep 27, 2008·Brain Research·Sandra RafalzikRüdiger Gerstberger
Nov 10, 2012·PloS One·Xiao-Fei GaoYan-Ai Mei
Nov 2, 2017·Neurological Research·Yuxiao ZhaoYonghua Ji
Feb 16, 2019·The Journal of Pharmacology and Experimental Therapeutics·John A BennettCharles J Lowenstein
Aug 20, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew G FisherJonathan C Jarvis
Feb 26, 2021·Circulation Research·Peter HannaKalyanam Shivkumar
May 4, 2021·Frontiers in Immunology·Namrita Halder, Girdhari Lal
Apr 19, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Srijan AcharyaKyeong-Man Kim
Aug 3, 2021·Experimental Cell Research·Pritam Kumar RoyMahitosh Mandal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.