Muscarinic autoreceptors modulate transmitter release through protein kinase C and protein kinase A in the rat motor nerve terminal

The European Journal of Neuroscience
Manel M SantafeJosep Tomàs

Abstract

We have used intracellular recording to investigate the existence of a functional link between muscarinic presynaptic acetylcholine (ACh) autoreceptors, the intracellular serine-threonine kinases-mediated transduction pathways and transmitter release in the motor nerve terminals of adult rats. We found the following. (1) Transmitter release was reduced by the M1 muscarinic acetylcholine receptor (mAChR) blocker pirenzepine and enhanced by the M2 blocker methoctramine. The unselective mAChR blocker atropine increased ACh release, which suggests the unmasking of another parallel release-potentiating mechanism. There are therefore two opposite, though finely balanced, M1-M2 mAChR-operated mechanisms that tonically modulate transmitter release. (2) Both M1 and M2 mechanisms were altered when protein kinase C (PKC), protein kinase A (PKA) or the P/Q-type calcium channel were blocked. (3) Both PKC and PKA potentiated release when they were specifically stimulated [with phorbol 12-myristate 13-acetate (PMA) and Sp-8-Br cAMPs, respectively], and both needed the P/Q channel. (4) In normal conditions PKC seemed not to be directly involved in transmitter release (the PKC blocker calphostin C did not reduce release), whereas PKA was couple...Continue Reading

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Citations

Sep 3, 2010·Pflügers Archiv : European journal of physiology·Itzchak Parnas, Hanna Parnas
Aug 8, 2008·Bulletin of Experimental Biology and Medicine·O P BalezinaS M Strukova
Sep 4, 2007·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M TakamoriH Yoshikawa
Aug 3, 2007·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·A I MalomouzhE E Nikolsky
Mar 29, 2014·Environmental Toxicology and Pharmacology·A F GodinhoC A Dias-Júnior
May 20, 2015·FEBS Letters·Yunhong Huang, Amantha Thathiah
Feb 2, 2016·Neurobiology of Aging·Anastasia ShakirzyanovaRashid Giniatullin
Mar 18, 2008·Journal of Neuropathology and Experimental Neurology·Neus GarciaJosep Tomàs
Aug 6, 2008·Journal of Neuroimmunology·Francisco J UrbanoOsvaldo D Uchitel
Dec 16, 2006·Trends in Neurosciences·Hanna Parnas, Itzchack Parnas
Sep 22, 2010·Journal of Neuroscience Research·Josep TomàsMarta Tomàs
Dec 21, 2012·Annals of the New York Academy of Sciences·Masaharu Takamori
Jan 28, 2014·Journal of Neuroscience Research·Josep TomàsErica Hurtado
Nov 26, 2009·The Journal of Comparative Neurology·Núria BesalduchMaria Angel Lanuza
Sep 10, 2014·Molecular Neurobiology·Tyler B TarrStephen D Meriney
Oct 9, 2014·Frontiers in Human Neuroscience·Mamede de CarvalhoMichael Swash
Oct 20, 2017·Korean journal of anesthesiology·Yong Beom KimHong Seuk Yang
Mar 31, 2007·Journal of Neuroscience Research·M M SantaféJ Tomàs
Oct 12, 2018·Journal of Applied Physiology·Ramon MargalefManel M Santafe
Feb 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Víctor Cilleros-MañéMaria Angel Lanuza
Apr 1, 2016·American Journal of Physiology. Renal Physiology·Wenkuan XinGeorgi V Petkov

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