Muscimol-induced death of GABAergic neurons in rat brain aggregating cell cultures

Brain Research. Developmental Brain Research
P HoneggerF Monnet-Tschudi

Abstract

During brain development, spontaneous neuronal activity has been shown to play a crucial role in the maturation of neuronal circuitries. Activity-related signals may cause selective neuronal cell death and/or rearrangement of neuronal connectivity. To study the effects of sustained inhibitory activity on developing inhibitory (GABAergic) neurons, three-dimensional primary cell cultures of fetal rat telencephalon were used. In relatively immature cultures, muscimol (10 microns), a GABAA receptor agonist, induced a transient increase in apoptotic cell death, as evidenced by a cycloheximide-sensitive increase of free nucleosomes and an increased frequency of DNA double strand breaks (TUNEL labeling). Furthermore, muscimol caused an irreversible reduction of glutamic acid decarboxylase activity, indicating a loss of GABAergic neurons. The muscimol-induced death of GABAergic neurons was attenuated by the GABAA receptor blockers bicuculline (100 microns) and picrotoxin (100 microns), by depolarizing potassium concentrations (30 mM KCl) and by the L-type calcium channel activator BAY K8644 (2 microns). As compared to the cholinergic marker (choline acetyltransferase activity), glutamic acid decarboxylase activity was significantly mor...Continue Reading

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Citations

Jun 15, 1999·Brain Research. Developmental Brain Research·B Pardo, P Honegger
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B Pardo, P Honegger
Oct 3, 2002·Journal of Neurochemistry·Marion MausJoel Premont
Jul 3, 2002·Experimental Neurology·Gail D ZeevalkPatricia K Sonsalla
Mar 14, 2008·Experimental Eye Research·Hideaki OkumichiTakashi Kanamoto
Jun 2, 2005·Trends in Neurosciences·Alfonso Represa, Yehezkel Ben-Ari
Jul 28, 2005·The European Journal of Neuroscience·Peng ZhaoYing Xia
Jul 29, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer RakotomamonjyAbdel M Ghoumari
May 23, 2000·Journal of Neurochemistry·K Lukasiuk, A Pitkänen
Sep 7, 2013·Oncology Reports·King-Chuen WuJing-Gung Chung

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis