Muscle-derived factors that support survival and promote fiber outgrowth from embryonic chick spinal motor neurons in culture

Developmental Biology
U DohrmannH Thoenen

Abstract

The purposes of the experiments reported is to provide an unambiguous demonstration that embryonic skeletal muscle contains factors that act directly on embryonic spinal motor neurons both to support their survival and to stimulate the outgrowth of neurites. Cells of lumbar and brachial ventral spinal cords from 6-day-old chick embryos were separated by centrifugation in a two-step metrizamide gradient, and a motor neuron enriched fraction was obtained. Motor neurons were identified by retrogradely labeling with rhodamine isothiocyanate, and were enriched fourfold in the motor neuron fraction relative to unfractionated cells. In culture, the isolated motor neurons died within 3-4 days unless they were supplemented with embryonic chick skeletal muscle extract. Two functionally distinct entities separable by ammonium sulfate precipitation were responsible for the effects of muscle extracts on motor neurons. The 0-25% ammonium sulfate precipitate contained molecules that alone had no effect on neuronal survival but when bound to polyornithine-coated culture substrata, stimulated neurite outgrowth and potentiated the survival activity present in muscle. Most of this activity was due to a laminin-like molecule being immunoprecipitat...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S MasukoY Shimada
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1976·The Journal of Comparative Neurology·M Hollyday, V Hamburger
Apr 15, 1975·The Journal of Comparative Neurology·V Hamburger
Jul 1, 1975·The Journal of Physiology·L Landmesser, D G Morris
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A D LanderL F Reichardt
Nov 1, 1970·Journal of Neurochemistry·H L White, C J Cavallito
Jan 1, 1983·Annual Review of Physiology·Y A BardeH Thoenen
Sep 1, 1984·The Journal of Cell Biology·E M BarthH Thoenen
Nov 1, 1984·Journal of Neurochemistry·G BarbinS Varon
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A D LanderL F Reichardt
Jul 29, 1983·Neuroscience Letters·K L Eagleson, M R Bennett
Jan 1, 1982·International Review of Cytology·T J Cunningham
May 1, 1983·Muscle & Nerve·J R SlackS Pockett
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C E HendersonJ P Changeux
Jul 3, 1980·Nature·T EbendalK O Hedlund

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Citations

Jun 1, 1990·Journal of Neuroscience Research·B H JuurlinkR M Devon
Feb 1, 1990·Journal of Neurocytology·A Kuecherer-EhretG W Kreutzberg
Oct 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M J Strong, R M Garruto
Jun 1, 1992·Journal of the Neurological Sciences·L C AngB H Juurlink
Dec 17, 1991·Brain Research. Developmental Brain Research·M W VogelK Herrup
May 13, 1994·Brain Research. Developmental Brain Research·P H BonnerR S Baker
Jul 1, 1989·Trends in Neurosciences·R W Oppenheim
Dec 1, 1990·Neuroscience Research·M Kuno
Aug 1, 1995·Progress in Neurobiology·M A Khan
Oct 28, 1991·Neuroscience Letters·T AmanoY Takahashi
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, A Vernadakis
Feb 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J P Ternaux, P Portalier
Oct 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P Ruiz-FlandesA C Kato
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·D Edgar
Aug 1, 1997·Neurochemistry International·E C KaalP R Bär
Mar 1, 1994·Journal of Voice : Official Journal of the Voice Foundation·S Bodine-Fowler
Jan 12, 1999·British Journal of Pharmacology·J P HubertA Doble
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G CorfasG D Fischbach
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·V E KoliatsosA Rosenthal
Nov 1, 1988·The Journal of Cell Biology·C Magill-Solc, U J McMahan
Jun 1, 1990·The Journal of Cell Biology·P V Sarthy, M Fu
Jul 1, 1991·The Journal of Cell Biology·A AchesonU Rutishauser

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