Muscle-derived stem cells used to treat skin defects prevent wound contraction and expedite reepithelialization

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Julia BujánJuan M Bellón

Abstract

Stem cells derived from adult tissues may serve as cell therapy to enhance the healing process in skin wounds. This study was designed to evaluate the use of autologous muscle-derived stem cells in an experimental skin wound model in terms of their efficiency at promoting tissue repair/regeneration. Muscle-derived cells obtained from the dorsal muscle of New Zealand rabbits were cultured in vitro for 2 weeks. The cell population was identified using the satellite markers CD34, m-cadherin and Myf5, and the proliferative capacity of the adult stem cells was determined. The population was then fluorescently labeled with PKH26 and seeded onto a circular 2 cm diameter defect created on the dorsal side of the ear of the rabbit from which the cells had been harvested. Similar defects on the contra lateral ears were left untreated to form the control group. Fourteen days later, specimens were taken for light, transmission, and scanning electron microscopy, as well as for immunolabeling with antibodies against vimentin, alpha-actin, desmin, myosin, fibronectin, and cytokeratin 14. Areas of wound contraction and reepithelialization were determined by image analysis. Wound contraction was significantly greater in the control than the trea...Continue Reading

References

Jan 1, 1990·Annals of Plastic Surgery·S T Ahn, T A Mustoe
Jul 1, 1995·Plastic and Reconstructive Surgery·W R OttoR Dover
Jan 9, 1999·Burns : Journal of the International Society for Burn Injuries·A MeanaF Tevar
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Jan 5, 2001·BMJ : British Medical Journal·P SerupT Mandrup-Poulsen
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yang D TengEvan Y Snyder
Sep 26, 2002·The British Journal of Surgery·D W Thomas, K G Harding
Jan 9, 2003·Lancet·Christof StammGustav Steinhoff
May 22, 2003·The International Journal of Biochemistry & Cell Biology·Jennifer E Morgan, Terence A Partridge
Jun 18, 2003·Journal of Cellular Physiology·Evangelos V BadiavasPeter Quesenberry
Dec 4, 2003·Experimental Cell Research·Monique BerendseCatriona M Lloyd
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Frank P Barry, J Mary Murphy
Mar 11, 2004·The International Journal of Biochemistry & Cell Biology·Stephen G BallCay M Kielty
Apr 28, 2004·Biochemical and Biophysical Research Communications·Satyakam Bhagavati, Weimin Xu

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Citations

Aug 1, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Cui-ping Zhang, Xiao-bing Fu
Dec 25, 2008·Regenerative Medicine·Martin J StoddartMauro Alini
Apr 3, 2007·Emergency Medicine Clinics of North America·Margaret K Strecker-McGrawDavid G Baer
Feb 14, 2008·American Journal of Physiology. Endocrinology and Metabolism·Klemen StrleKeith W Kelley
Aug 15, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ksymena UrbanekAleksander L Sieroń

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