Muscle protein analysis by two-dimensional gel electrophoresis

Critical Reviews in Clinical Laboratory Sciences
C S Giometti

Abstract

Two-dimensional electrophoresis was first applied to the analysis of muscle proteins in 1976 when the occurrence of multiple forms of actin was discovered. Since that time the technique has become widely accepted as a new approach to studies of myogenesis, muscle differentiation, and muscle pathology. In addition, two-dimensional electrophoresis now is being used to investigate contractile proteins present in nonmuscle cells. This review will discuss, in general, the technique of two-dimensional electrophoresis in polyacrylamide gels which combines isoelectric focusing and sodium dodecyl sulfate electrophoresis. The application of the technique specifically to muscle protein analysis will be discussed through a review of existing literature on two-dimensional electrophoresis of cultured muscle cells and tissue homogenates. Attention will be given to contractile protein heterogeneities such as alpha, beta, and gamma actin and the embryonic forms of myosin light chains, all discovered through the use of two-dimensional electrophoresis. New information concerning gene expression during muscle differentiation revealed by differences in two-dimensional electrophoresis protein patterns and the use of two-dimensional electrophoresis f...Continue Reading

References

Jun 1, 1978·Cell·E Lazarides, D R Balzer
Jan 1, 1978·Differentiation; Research in Biological Diversity·S A FelliniH Holtzer
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C R MerrilM L Van Keuren
Feb 1, 1979·Developmental Biology·E A Fyrberg, J J Donady
Nov 1, 1979·Molecular & General Genetics : MGG·C Y LeeL Bennett
Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Dec 14, 1979·Biochimica Et Biophysica Acta·L D LiberaS Schiaffino
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J G Izant, E Lazarides
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A MakM Bárány
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C M O'ConnorE Lazarides
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D L GardE Lazarides
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E H McConkeyD Phan
Apr 14, 1978·JAMA : the Journal of the American Medical Association·T Furukawa, J B Peter
Apr 21, 1978·JAMA : the Journal of the American Medical Association·T Furukawa, J B Peter
Jul 6, 1978·Nature·G F GauthierA W Hobbs
Mar 1, 1979·Developmental Biology·R B Devlin, C P Emerson
Jul 1, 1979·Journal of the Neurological Sciences·I H FraserC C Liew
Nov 1, 1979·Developmental Biology·J I Garrels
Jan 31, 1979·Pflügers Archiv : European journal of physiology·D PetteR G Whalen
Apr 25, 1975·Journal of Molecular Biology·C P Emerson, S K Beckner
Feb 1, 1977·Archives of Biochemistry and Biophysics·H RibolowM Bárány
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J P MerlieF Gros
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R G WhalenF Gros
May 19, 1975·Biochemical and Biophysical Research Communications·E Gruenstein, A Rich
Jan 1, 1973·Journal of the Neurological Sciences·M A JohnsonD Appleton
Mar 1, 1971·The Journal of Physiology·M Bárány, R I Close
Sep 1, 1971·Developmental Biology·D Fambrough, J E Rash
May 1, 1971·Archives of Biochemistry and Biophysics·A Martonosi, R A Halpin
Oct 10, 1973·Nature: New Biology·R E FineB Kaminer
Sep 1, 1971·Developmental Biology·I R Konigsberg
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J M Prives, B Paterson
Oct 1, 1972·Developmental Biology·B Paterson, R C Strohman
Sep 1, 1973·The Biochemical Journal·W T PerrieS B Perry
Aug 1, 1973·Developmental Biology·B W WilsonD M Fry
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jul 21, 1972·Journal of Molecular Biology·I Cohen, C Cohen
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Jun 14, 1968·Annals of the New York Academy of Sciences·D M DawsonM E Eppenberger
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L R Keller, C P Emerson
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F E StockdaleH Baden
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C WangE Lazarides
Aug 6, 1981·Nature·B F Clark
Mar 1, 1980·Experimental Neurology·J H SteinbachL Eldridge
Aug 1, 1980·Experimental Cell Research·H BrzeskiN R Ringertz
Jul 16, 1980·Biochemical and Biophysical Research Communications·R BretonF Gros
Sep 16, 1980·Biochemical and Biophysical Research Communications·R A JanisR T Gualtieri
Sep 15, 1971·FEBS Letters·P M. Sender

Citations

Aug 1, 1985·Annals of Neurology·C S Giometti, M J Danon
Mar 19, 2011·Journal of Proteomics·Cecilia GelfiPaolo Cerretelli
Sep 28, 2006·Clinical and Experimental Pharmacology & Physiology·Kym J GuelfiPeter G Arthur
Nov 20, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dalila Laoudj-ChenivesseAnne Fernandez
Sep 13, 1991·Journal of Chromatography·T MarshallK M Williams

Related Concepts

Isoactin
Cell Differentiation Process
SDS-PAGE
Isoelectric Focusing
Muscle Proteins
Muscle
Myopathy
Actomyosin Adenosinetriphosphatase
Phosphorylation
Miniparamyosin

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M BurkeW F Harrington
Biochemical and Biophysical Research Communications
L Dalla LiberaS Schiaffino
© 2021 Meta ULC. All rights reserved