PMID: 2123467Oct 1, 1990Paper

Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, and Sp1

Genes & Development
V SartorelliL Kedes

Abstract

Expression of the human cardiac alpha-actin gene (HCA) depends on the interactions of multiple transcriptional regulators with promoter elements. We report here that the tissue-specific expression of this promoter is determined by the simultaneous interaction of at least three specific protein-DNA complexes. The myogenic determinant gene MyoD1 activated the transcription of transfected HCA-CAT promoter constructs in nonmuscle cells, including CV-1 and HeLa cells. Gel mobility-shift and footprinting assays revealed that MyoD1 specifically interacted with a single consensus core sequence, CANNTG, at -50. Previously characterized sites interact with a protein identical with or related to the serum response factor (SRF) at -100 and Sp1 at -70. All three elements must be intact to support transcription in muscle cells: site-specific mutation within any one of these three elements eliminated transcriptional expression by the promoter. Furthermore, expression of the promoter in embryonic Drosophila melanogaster cells that lack MyoD1 and Sp1 is strictly dependent on all three sites remaining intact and on the presence of exogenously supplied Sp1 and MyoD1. These experiments suggest that the presence of three sequence-specific binding p...Continue Reading

References

Jan 1, 1977·Differentiation; Research in Biological Diversity·D Yaffe, O Saxel
Dec 1, 1989·Current Opinion in Cell Biology·R E Kingston
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WeintraubA D Miller
Nov 4, 1988·Cell·T Curran, B R Franza
Aug 25, 1988·Nature·G Gill, M Ptashne
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T MiwaL Kedes

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Citations

Aug 26, 1998·Journal of Cellular Biochemistry·J GraysonR S Williams
Jan 1, 1996·Molecular Reproduction and Development·L LiangG K Andrews
Jan 1, 1996·Developmental Genetics·J A Dominov, J B Miller
Mar 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A FaermanM Shani
Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J B MillerJ A Dominov
Oct 1, 1992·Journal of Cellular Biochemistry·F ColeD S Kohtz
Aug 1, 1991·Molecular Reproduction and Development·J Varley, S Brennan
Feb 7, 2001·Biochemical and Biophysical Research Communications·K KuroharaA Fujisawa-Sehara
Dec 31, 2002·Journal of Molecular and Cellular Cardiology·Qin WangJim Jung-Ching Lin
Mar 1, 1993·Somatic Cell and Molecular Genetics·T PrigozyC Shuler
Jan 1, 1993·Molecular Neurobiology·R G KallenR L Barchi
Apr 1, 1996·The International Journal of Biochemistry & Cell Biology·P A Doevendans, M van Bilsen
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Joseph M Miano
Jan 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stryder M MeadowsPaul A Krieg
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M E Van AntwerpE V Prochownik
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·V SartorelliL Kedes
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·E BengalI M Verma
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L BessereauJ P Changeux
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J D Molkentin, E N Olson
Mar 10, 2009·The Journal of Biological Chemistry·Helen P MakarenkovaRobyn Meech
Jun 1, 1995·The Journal of Cell Biology·C Murphy-ErdoshL F Reichardt
Apr 9, 2011·Antioxidants & Redox Signaling·Yukihiro SaitoTadahiro Sasajima
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer

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