Aug 15, 2018

Muscle Stem Cell Immunostaining

Current Protocols in Mouse Biology
Shuaiyu WangHongbo Zhang

Abstract

Muscle stem cells (MuSCs) are essential for maintaining muscle homeostasis by providing progenitor cells for muscle regeneration after injury and in muscular diseases. MuSC properties dynamically change, reflecting physiology or pathological status. For instance, MuSCs are activated after muscle injury, but become exhausted in late stages of Duchenne Muscular Dystrophy (DMD) disease and senescent during aging. Therefore, characterization of MuSCs, including proliferation, activation, senescence, and apoptosis, etc., is very important in applying MuSC knowledge to regenerative medicine, such as in the treatment of DMD and to improve muscle function in aging. Here, we describe a detailed method for characterizing MuSCs in situ using immunostaining techniques in the mouse. This method can also be easily adapted to analyze other skeletal muscle properties. © 2018 by John Wiley & Sons, Inc.

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Mentioned in this Paper

Myoblasts
Regenerative Medicine
Myopathy
Soleus Muscle Structure
Muscle Satellite Cell
Cell Proliferation
Muscle Function
Regenerating Muscle Fiber
Senescence Function
Apoptosis

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