Mushroom Body Specific Transcriptome Analysis Reveals Dynamic Regulation of Learning and Memory Genes After Acquisition of Long-Term Courtship Memory in Drosophila

G3 : Genes - Genomes - Genetics
Spencer G JonesJamie M Kramer

Abstract

The formation and recall of long-term memory (LTM) requires neuron activity-induced gene expression. Transcriptome analysis has been used to identify genes that have altered expression after memory acquisition, however, we still have an incomplete picture of the transcriptional changes that are required for LTM formation. The complex spatial and temporal dynamics of memory formation creates significant challenges in defining memory-relevant gene expression changes. The Drosophila mushroom body (MB) is a signaling hub in the insect brain that integrates sensory information to form memories across several different experimental memory paradigms. Here, we performed transcriptome analysis in the MB at two time points after the acquisition of LTM: 1 hr and 24 hr. The MB transcriptome was compared to biologically paired whole head (WH) transcriptomes. In both, we identified more transcript level changes at 1 hr after memory acquisition (WH = 322, MB = 302) than at 24 hr (WH = 23, MB = 20). WH samples showed downregulation of developmental genes and upregulation of sensory response genes. In contrast, MB samples showed vastly different changes in transcripts involved in biological processes that are specifically related to LTM. MB-dow...Continue Reading

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Citations

Nov 7, 2018·G3 : Genes - Genomes - Genetics·Meng-Fu Maxwell ShihJosh Dubnau
Jun 27, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenifer C Kaldun, Simon G Sprecher
Feb 2, 2021·Journal of Neurogenetics·Nicholas RaunJamie M Kramer
Apr 17, 2021·Frontiers in Behavioral Neuroscience·Camilla RoselliIsaac Cervantes-Sandoval

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Datasets Mentioned

BETA
GSE115718

Methods Mentioned

BETA
DamID
RNA-seq
PCA
immunoprecipitation

Software Mentioned

count
geneMANIA
DESeq2
PANTHER
R
DamID
HTSeq
NOISeq
Image J
Cytoscape

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