Mussel adhesive protein provides cohesive matrix for collagen type-1α

Biomaterials
Nadine R Martinez RodriguezJ Herbert Waite

Abstract

Understanding the interactions between collagen and adhesive mussel foot proteins (mfps) can lead to improved medical and dental adhesives, particularly for collagen-rich tissues. Here we investigated interactions between collagen type-1, the most abundant load-bearing animal protein, and mussel foot protein-3 (mfp-3) using a quartz crystal microbalance and surface forces apparatus (SFA). Both hydrophilic and hydrophobic variants of mfp-3 were exploited to probe the nature of the interaction between the protein and collagen. Our chief findings are: 1) mfp-3 is an effective chaperone for tropocollagen adsorption to TiO2 and mica surfaces; 2) at pH 3, collagen addition between two mfp-3 films (Wc = 5.4 ± 0.2 mJ/m(2)) increased their cohesion by nearly 35%; 3) oxidation of Dopa in mfp-3 by periodate did not abolish the adhesion between collagen and mfp-3 films, and 4) collagen bridging between both hydrophilic and hydrophobic mfp-3 variant films is equally robust, suggesting that hydrophobic interactions play a minor role. Extensive H-bonding, π-cation and electrostatic interactions are more plausible to explain the reversible bridging of mfp-3 films by collagen.

References

Apr 6, 2001·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·K RiechertP Sinha
Sep 19, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jyrki Heino
Apr 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Travers H AndersonJacob N Israelachvili
Apr 27, 2011·Advanced Materials·Jing YuJ Herbert Waite
May 24, 2011·Advanced Functional Materials·Travers H AndersonJacob N Israelachvili
Sep 8, 2011·European Journal of Oral Sciences·Raquel OsorioManuel Toledano
Nov 8, 2011·Annual Review of Materials Research·Bruce P LeeJ H Waite
Aug 10, 2012·Biochemistry·Eric W DannerJ Herbert Waite
Sep 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jing YuJacob N Israelachvili
Sep 26, 2013·Acta Biomaterialia·Wei WeiJ Herbert Waite
Oct 10, 2013·Biotechnology Progress·Sascha C T NicklischJacob N Israelachvili

❮ Previous
Next ❯

Citations

Apr 16, 2015·Biofouling·Nadine R Martinez RodriguezJ Herbert Waite
Feb 19, 2015·Biomacromolecules·Saurabh DasJ Herbert Waite
Mar 7, 2017·Physical Chemistry Chemical Physics : PCCP·Marta CornoPiero Ugliengo
Oct 20, 2015·Nature Communications·B Kollbe AhnJ Herbert Waite
Jun 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yiran LiYi Cao
Sep 23, 2015·Advanced Functional Materials·Saurabh DasJacob N Israelachvili
Feb 14, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Kang LiCynthia Kar Yung Yiu
Jun 12, 2020·Journal of Dentistry·Kang LiCynthia Kar Yung Yiu
Aug 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ameya R NarkarKollbe Ahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.