Mutagen formation in a model beef supernatant fraction. IV. Properties of the system

Environmental Health Perspectives
R T TaylorM G Knize

Abstract

To identify the precursors and elucidate the reaction conditions that yield heterocyclic amine mutagens in cooked meat products and fish, we have used a supernatant 2 (S2) fraction prepared from H2O-homogenized lean round steak. Compounds (MW less than 500) in S2 are the sources of the microsomal-dependent, Salmonella TA 1538 mutagenic activity in open boiled (aqueous), 200 degrees C pressure-heated (aqueous), or 200 to 300 degrees C oven-baked (freeze-dried) homogenates. Combined incorporation-HPLC experiments show that they are also the precursors for frameshift mutagen formation in the outer surfaces of 200 degrees C griddle-fried ground beef. Maximal stimulations of boiled S2 mutagenic activity are given by 10 mM Trp, 2.5 mM creatine phosphate (CP), and synergistically by 10 mM Trp + 2.5 mM CP + 1.0 mM FeSO4 (a mixture abbreviated as S2*). Boiling S2 for 30 hr at the acidic optimum pH of 4.0----600 TA 1538 revertants (no additions) and 1,400 revertants (+CP), while S2*----24,000 revertants/10(8) bacteria/g of dry beef. By the criteria of HPLC, paper electrophoresis, and resistance of the active HPLC fractions to acid-nitrite inactivation, boiled S2 contains 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 3-amino-1-methyl-5H-p...Continue Reading

References

Sep 1, 1978·Mutation Research·D Yoshida, T Matsumoto
Sep 1, 1979·Cancer Letters·N E Spingarn, J H Weisburger
Dec 1, 1983·Mutation Research·K ShinoharaH Omura
Mar 1, 1984·Mutation Research·E OvervikJ A Gustafsson
Jan 1, 1984·Carcinogenesis·J S FeltonF T Hatch
Aug 1, 1982·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L F BjeldanesF T Hatch
Jan 1, 1983·Journal of Agricultural and Food Chemistry·L F BjeldanesF T Hatch
Oct 1, 1982·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J Y LinH I Huang
Jan 1, 1982·Annals of the New York Academy of Sciences·L Reid, B Meyrick
Mar 1, 1980·Journal of Agricultural and Food Chemistry·T Shibamoto

❮ Previous
Next ❯

Citations

Mar 1, 1996·Environmental Health Perspectives·S Robbana-BarnatJ Fradin
Mar 9, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A SolyakovM Jägerstad
Oct 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J S FeltonM G Knize
Dec 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Johansson, M Jägerstad
Sep 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K Skog
Sep 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D J DoolittleA W Hayes
Dec 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M G KnizeJ S Felton
Jan 1, 1992·Free Radical Biology & Medicine·A M PearsonS D Aust
Oct 1, 1994·Environmental Health Perspectives·J S FeltonR Wu
Jan 1, 1988·Nutrition and Cancer·V E Archer
Oct 1, 1993·Environmental Monitoring and Assessment·A D Lemly
Dec 20, 1999·Journal of Agricultural and Food Chemistry·P PaisJ S Felton
Oct 13, 2006·Journal of Agricultural and Food Chemistry·Rosa BusquetsKerstin Skog
May 23, 2000·Journal of Agricultural and Food Chemistry·P PaisM G Knize

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
M G KnizeJ S Felton
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
L F BjeldanesF T Hatch
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
K Skog
© 2021 Meta ULC. All rights reserved