PMID: 377021Apr 17, 1979Paper

Mutagenesis and repair deficiencies of Escherichia coli umuC mutants are suppressed by the plasmid pKM101

Molecular & General Genetics : MGG
G C Walker, P P Dobson

Abstract

The presence of the drug resistance plasmid pKM101 restored the ability of Escherichia coli umuC mutant strains to be mutated by methyl methanesulfonate. Inducible (Weigle) reactivation of ultraviolet-irradiated bacteriophage lambda was not observed in uvrA6 umuC mutant strains lacking pKM101 but was observed if the plasmid was present in the strains. In a uvrA+ umuC36 strain pKM101 increased the efficiency of the Weigle reactivation process. Plasmid-mediated UV-resistance and plasmid-mediated phage reactivation were observed in umuC(pKM101) strains both in uvrA+ and uvrA6 backgrounds. No restoration of methyl methanesulfonate mutability by pKM101 was observed in umuC36 recA56 strains. pKM101 mutants unable to enhance mutagenesis in umuC+ backgrounds also had no effect on methyl methanesulfonate mutagenesis in umuC mutant strains. Neither a umuC mutation nor the presence of pKM101 affected the UV induction of protein X, the recA protein. Hypotheses relating the mode of action of pKM101 to the process of mutagenesis and inducible phage reactivation are discussed.

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Citations

Oct 1, 1991·Molecular & General Genetics : MGG·S G SedgwickP Strike
Jan 1, 1981·Molecular & General Genetics : MGG·P J Langer, G C Walker
Jan 1, 1981·Molecular & General Genetics : MGG·J Hofemeister, G Eitner
Jan 1, 1985·Molecular & General Genetics : MGG·A Brotcorne-LannoyeM Radman
Jan 1, 1984·Molecular & General Genetics : MGG·S B DowdenP Strike
Aug 1, 1989·Molecular & General Genetics : MGG·S G SedgwickP Strike
Jan 1, 1982·Molecular & General Genetics : MGG·Z Cieśla
Jan 1, 1984·Molecular & General Genetics : MGG·V M KopylovM N Kolot
Jan 1, 1983·Molecular & General Genetics : MGG·M Defais
Jan 1, 1981·Molecular & General Genetics : MGG·K McCann, C H Clarke
Jan 1, 1980·Molecular & General Genetics : MGG·W G Shanabruch, G C Walker
Jan 1, 1985·Molecular & General Genetics : MGG·J JenekM Hofnung
Nov 1, 1980·Mutation Research·R BockrathS Kristoff
Nov 1, 1982·Mutation Research·R M SchaaperL A Loeb
Jan 1, 1987·Mutation Research·J M ParamioR de Vidania
Jan 1, 1989·Mutation Research·S K GhoshJ Das
Nov 16, 1992·Mutation Research·V SlezárikováA G Skavronskaya
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Feb 1, 1981·Mutation Research·G WarrenS Rogers
Aug 1, 1993·Mutation Research·M Altshuler
Dec 13, 2000·Mutation Research·K Mortelmans, E Zeiger
May 9, 2001·Mutation Research·G C Walker
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Jun 7, 2007·Nature Reviews. Molecular Cell Biology·Katharina Schlacher, Myron F Goodman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C J Kenyon, G C Walker

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