Mutagenesis approaches for elucidation of protein structure-function relationships

Current Protocols in Neuroscience
C D Strader

Abstract

Several mutagenesis strategies have been used to allow the identification of specific receptor-ligand interactions to support the docking of ligands within various receptor models. The strengths and limitations of each strategy are discussed in this overview. Large-scale mapping is described using deletion and chimeric receptors. Fine tuning with single residue replacements is also covered along with two-dimensional approaches.

References

Jan 1, 1994·Annual Review of Biochemistry·C D StraderR A Dixon
Apr 22, 1993·Nature·G F SchertlerR Henderson

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Citations

Jul 3, 2021·International Journal of Molecular Sciences·Kuan-Hsi Chen, Yuh-Jyh Hu

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