Mutagenesis of the C1 oxidation pathway in Methanosarcina barkeri: new insights into the Mtr/Mer bypass pathway

Journal of Bacteriology
Paula V Welander, William W Metcalf

Abstract

A series of Methanosarcina barkeri mutants lacking the genes encoding the enzymes involved in the C1 oxidation/reduction pathway were constructed. Mutants lacking the methyl-tetrahydromethanopterin (H4MPT):coenzyme M (CoM) methyltransferase-encoding operon (delta mtr), the methylene-H4MPT reductase-encoding gene (delta mer), the methylene-H4MPT dehydrogenase-encoding gene (delta mtd), and the formyl-methanofuran:H4MPT formyl-transferase-encoding gene (delta ftr) all failed to grow using either methanol or H2/CO2 as a growth substrate, indicating that there is an absolute requirement for the C1 oxidation/reduction pathway for hydrogenotrophic and methylotrophic methanogenesis. The mutants also failed to grow on acetate, and we suggest that this was due to an inability to generate the reducing equivalents needed for biosynthetic reactions. Despite their lack of growth on methanol, the delta mtr and delta mer mutants were capable of producing methane from this substrate, whereas the delta mtd and delta ftr mutants were not. Thus, there is an Mtr/Mer bypass pathway that allows oxidation of methanol to the level of methylene-H4MPT in M. barkeri. The data further suggested that formaldehyde may be an intermediate in this bypass; howe...Continue Reading

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Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gargi KulkarniWilliam W Metcalf
Jan 1, 2012·ISRN Microbiology·Joshua T EllisCharles D Miller
May 13, 2009·Molecular Microbiology·Ellen Oelgeschläger, Michael Rother
Feb 20, 2013·Biotechnology Journal·Matthew C GonnermanNathan D Price
May 14, 2011·Molecular Microbiology·Michael J HohnDieter Söll
Nov 3, 2009·Environmental Microbiology·Anke MeyerdierksRudolf Amann
Sep 18, 2010·Archaea : an International Microbiological Journal·Michael Rother, Joseph A Krzycki
May 18, 2016·Genome Biology and Evolution·Guillaume BorrelSimonetta Gribaldo
Feb 23, 2018·Environmental Microbiology·Viola KrukenbergGunter Wegener
Jan 22, 2019·Nature Reviews. Microbiology·Paul N EvansGene W Tyson
Jan 6, 2018·AMB Express·Franziska EnzmannDirk Holtmann
Feb 6, 2017·Archaea : an International Microbiological Journal·Peer H A TimmersAlfons J M Stams
Jan 17, 2021·Applied and Environmental Microbiology·Jared AldridgeNicole R Buan

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