PMID: 9420267Jan 7, 1998Paper

Mutagenesis of the NS3 protease of dengue virus type 2

Journal of Virology
R P Valle, B Falgout


The flavivirus protease is composed of two viral proteins, NS2B and NS3. The amino-terminal portion of NS3 contains sequence and structural motifs characteristic of bacterial and cellular trypsin-like proteases. We have undertaken a mutational analysis of the region of NS3 which contains the catalytic serine, five putative substrate binding residues, and several residues that are highly conserved among flavivirus proteases and among all serine proteases. In all, 46 single-amino-acid substitutions were created in a cloned NS2B-NS3 cDNA fragment of dengue virus type 2, and the effect of each mutation on the extent of self-cleavage of the NS2B-NS3 precursor at the NS2B-NS3 junction was assayed in vivo. Twelve mutations almost completely or completely inhibited protease activity, 9 significantly reduced it, 14 decreased cleavage, and 11 yielded wild-type levels of activity. Substitution of alanine at ultraconserved residues abolished NS3 protease activity. Cleavage was also inhibited by substituting some residues that are conserved among flavivirus NS3 proteins. Two (Y150 and G153) of the five putative substrate binding residues could not be replaced by alanine, and only Y150 and N152 could be replaced by a conservative change. The...Continue Reading


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Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
Nov 1, 1990·Nature·B MossT R Fuerst
Jun 1, 1990·Virology·K Osatomi, H Sumiyoshi
May 25, 1989·Nucleic Acids Research·A E GorbalenyaV M Blinov
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
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