Mutagenesis of the virion-sense open reading frames of tomato leaf curl geminivirus

Virology
J E RigdenM A Rezaian

Abstract

A series of frame shift, deletion, and inversion mutants in the virion-sense open reading frames (ORFs) of the monopartite geminivirus tomato leaf curl virus have been constructed and their ability to replicate, produce single-stranded DNA, spread, and cause symptoms in tomato plants has been investigated. Disruptions in the V1 ORF lead to symptomless, systemic infections with a reduced titer of all viral DNA forms while interruptions in the V2 (coat protein) ORF disrupted spread of the virus. Mutagenesis of the virion-sense ORFs did not affect the replication of viral double-stranded DNA, although both V1 and V2 products appear to play a role in the accumulation of viral single-stranded DNA.

Citations

Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·T KunikY Gafni
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E RigdenM A Rezaian
Jul 15, 2011·Molecular Plant-microbe Interactions : MPMI·Imran AminRob W Briddon
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Luke A SelthM Ali Rezaian
Aug 5, 2005·Annual Review of Phytopathology·Maria R RojasRobert L Gilbertson
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·I B DryM A Rezaian
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·K SaundersJ Stanley
Jan 1, 2003·Molecular Plant Pathology·Yedidya Gafni
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·I DryA Rezaian
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
Jun 10, 2011·Journal of Biosciences·A RouhibakhshV G Malathi
May 31, 2019·Virusdisease·Rashmi Rishishwar, Indranil Dasgupta

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