PMID: 2117468Jun 12, 1990Paper

Mutagenic, electrochemical, and crystallographic investigation of the cytochrome b5 oxidation-reduction equilibrium: involvement of asparagine-57, serine-64, and heme propionate-7

Biochemistry
W D FunkA G Mauk

Abstract

A gene coding for lipase-solubilized bovine liver microsomal cytochrome b5 has been synthesized, expressed in Escherichia coli, and mutated at functionally critical residues. Characterization of the recombinant protein revealed that it has a reduction potential that is approximately 17 mV lower than that of authentic wild-type protein at pH 7 (25 degrees C). Structural studies determined that the recombinant protein differed in sequence from authentic wild-type cytochrome b5 owing to three errors in amidation status in the published sequence for the protein on which the gene synthesis was based. The structural origin of the lower reduction potential exhibited by the triple mutant has been investigated through X-ray crystallographic determination of the three-dimensional structure of this protein and is attributed to the presence of Asp-57 within 3.3 A of heme vinyl-4 in the mutant. In addition, the model developed by Argos and Mathews [Argos, P., & Mathews, F.S. (1975) J. Biol. Chem. 250, 747] for the change in cytochrome b5 oxidation state has been studied through mutation of Ser-64 to Ala. In this model, Ser-64 is postulated to stabilize the oxidized protein through H-bonding interactions with heme propionate-7 that orients t...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 20, 1989·Journal of Molecular Biology·C J LeungG D Brayer
Jul 27, 1989·Biochimica Et Biophysica Acta·J Ozols
Jan 20, 1988·Journal of Molecular Biology·G V LouieG D Brayer
Jan 25, 1989·Nucleic Acids Research·R J Cristiano, A W Steggles
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B A Springer, S G Sligar
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S Beck von BodmanS G Sligar
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S SheriffL H Jensen
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Jan 1, 1985·Progress in Biophysics and Molecular Biology·F S Mathews
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·F S MathewsM Levine
Mar 14, 1972·Journal of Molecular Biology·F S MathewsP Argos
Apr 1, 1969·Journal of Molecular Biology·F S Mathews, P Strittmatter
Jan 1, 1980·Methods in Enzymology·G Chaconas, J H van de Sande
Nov 25, 1981·Journal of Molecular Biology·T Takano, R E Dickerson
Jan 1, 1983·Methods in Enzymology·J Messing
May 11, 1982·Nucleic Acids Research·G D StormoL M Gold
Apr 25, 1980·Biochimica Et Biophysica Acta·F S Mathews
Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Jan 1, 1986·Biophysical Journal·B C Finzel, F R Salemme
Aug 1, 1988·Journal of the American Chemical Society·F A WalkerD H Buttlaire

❮ Previous
Next ❯

Citations

Apr 17, 2007·Journal of Biosciences·S Rezaei-ZarchiA Javed
Jan 1, 1994·Biochimie·F Lederer
Apr 16, 2010·Journal of the American Chemical Society·Judith M NocekBrian M Hoffman
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P D BarkerA G Mauk
Apr 1, 1994·Journal of Biomolecular Structure & Dynamics·D S GillR C Willson
Apr 15, 1997·European Journal of Biochemistry·G P KellyD Whitford
Jun 30, 2000·Biochemical and Biophysical Research Communications·S SilchenkoM Rivera
Mar 27, 2001·Biochemical and Biophysical Research Communications·S HirotaZ X Huang
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian M HoffmanJudith M Nocek
Sep 6, 2007·Drug Metabolism Reviews·Michael A NobleAndrew W Munro
Nov 25, 2006·Biochimica Et Biophysica Acta·Jirí HudecekPavel Anzenbacher
Aug 6, 2004·Protein Science : a Publication of the Protein Society·Aaron B CowleyDavid R Benson
Feb 4, 2005·Protein Science : a Publication of the Protein Society·Alexander N VolkovMarcellus Ubbink
Aug 25, 2015·Biochimica Et Biophysica Acta·Parisa Hosseinzadeh, Yi Lu
Jul 7, 2015·Acta Crystallographica. Section D, Biological Crystallography·Yu HiranoTaro Tamada
Jun 22, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ying-Wu LinLi-Fu Liao
Feb 16, 2008·Biochimica Et Biophysica Acta·Liana V BasovaValentina E Bychkova
Jun 2, 2000·Protein Expression and Purification·S B Mulrooney, L Waskell
Mar 10, 2015·Biochemistry. Biokhimii︠a︡·V E BychkovaV A Balobanov
Jul 3, 2017·Biochimica Et Biophysica Acta. Proteins and Proteomics·Piotr J Mak, Ilia G Denisov
Oct 3, 2018·Biochemistry·Bezalel A BaconElizabeth M Boon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.