Mutagenic insertion and chromosome engineering resource (MICER)

Nature Genetics
David J AdamsA Bradley

Abstract

Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.

References

Dec 14, 1995·Nature·R Ramírez-SolisA Bradley
Oct 5, 2001·Nature Reviews. Genetics·Y Yu, A Bradley
Oct 10, 2001·Genome Research·Z NingJ C Mullikin
Nov 15, 2002·Cancer Biology & Therapy·Binhai ZhengAllan Bradley
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Mar 11, 2003·Nature Biotechnology·Giuseppe TestaA Francis Stewart
Apr 16, 2003·Nature·Francis S CollinsUNKNOWN US National Human Genome Research Institute
Jul 23, 2003·Genesis : the Journal of Genetics and Development·Ichiko NishijimaAllan Bradley
Dec 19, 2003·Nucleic Acids Research·E BirneyT Hubbard
Dec 19, 2003·Nucleic Acids Research·Carol J BultUNKNOWN Mouse Genome Database Group
Jan 7, 2004·Genomics·Jan KlysikAllan Bradley
May 29, 2004·Nature Genetics·William C SkarnesUNKNOWN International Gene Trap Consortium

❮ Previous
Next ❯

Citations

Nov 15, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Gareth R HowellJohn C Schimenti
Jan 16, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Erin PaulJan E Klysik
Jun 1, 2010·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Tao YuY Eugene Yu
Jul 10, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Olga ErmakovaCornelius Gross
Mar 29, 2011·Human Genetics·Chunhong LiuY Eugene Yu
Sep 9, 2005·Transgenic Research·Lisa E OlsonRoger H Reeves
Nov 26, 2009·Transgenic Research·Erin PaulJan E Klysik
Aug 24, 2004·Nature Genetics·Jiangzhen LiWei-Wen Cai
Oct 29, 2005·Nature Genetics·Stefan GlaserA Francis Stewart
Dec 8, 2009·Nature Medicine·Jillian L BarlowAndrew N J McKenzie
Feb 6, 2007·Nature Methods·Mélanie BilodeauGuy Sauvageau
Jul 5, 2005·Nature Reviews. Genetics·Corey M Carlson, David A Largaespada
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guy HorevAlea A Mills
Mar 20, 2009·Human Molecular Genetics·Frances K WisemanElizabeth M C Fisher
Feb 5, 2010·Human Molecular Genetics·Y Eugene YuJohn K Cowell
Feb 9, 2005·Nucleic Acids Research·Chunfang ZhangJohn R Morrison
May 29, 2008·Molecular and Cellular Biology·Ulrike C LangeM Azim Surani
Jul 11, 2006·Annual Review of Genomics and Human Genetics·Louise van der Weyden, Allan Bradley
Jul 19, 2005·Annual Review of Neuroscience·Mary E Hatten, Nathaniel Heintz
Jun 19, 2008·Physiological Genomics·David J Adams, Louise van der Weyden
May 13, 2010·BMC Genomics·Giulietta M Spudich, Xosé M Fernández-Suárez
Jul 14, 2009·PloS One·J Susie ZoltewiczAndrew S Peterson
Jul 19, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristi ClarkGrace M Hobson
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Angshumoy Roy, Martin M Matzuk
May 14, 2014·Nature Communications·Wangzhi LiAlea A Mills
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei WangAllan Bradley
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Roland H FriedelMarc Tessier-Lavigne
Aug 29, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Anna M MigdalskaDavid J Adams
Oct 21, 2009·The Journal of Cell Biology·Preethi VijayarajThomas M Magin
Oct 21, 2009·The Journal of Cell Biology·Juliane C Kellner, Pierre A Coulombe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.