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Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: deletion of gal7

Methods in Molecular Biology

Dec 7, 2011

Nikhil U Nair, Huimin Zhao

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Abstract

MIRAGE is a unique in vivo genome editing technique that exploits the inherent instability of inverted repeats (palindromes) in the Saccharomyces cerevisiae chromosome. As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications tha...read more

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
LGALS7 wt Allele
Transformation, Genetic
LGALS7B wt Allele
Saccharomyces cerevisiae allergenic extract
Hairpin Loop Sequence
Gene Deletion Abnormality
Gene Deletion
Homologous Recombination
LGALS7 gene
1
14
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Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: deletion of gal7

Methods in Molecular Biology

Dec 7, 2011

Nikhil U Nair, Huimin Zhao

PMID: 22144353

DOI: 10.1007/978-1-61779-483-4_5

Abstract

MIRAGE is a unique in vivo genome editing technique that exploits the inherent instability of inverted repeats (palindromes) in the Saccharomyces cerevisiae chromosome. As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications tha...read more

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
LGALS7 wt Allele
Transformation, Genetic
LGALS7B wt Allele
Saccharomyces cerevisiae allergenic extract
Hairpin Loop Sequence
Gene Deletion Abnormality
Gene Deletion
Homologous Recombination
LGALS7 gene
1
14

Feeds With Similar Papers

Chromosomal Deletion

Chromosomal deletion includes the loss of a gene sequence of DNA. The location and the genes deleted determines the significance of this abnormality. There are many identified genetic disorders that are a result from chromosomal deletion including cri du chat and Prader-Willi syndrome. Discover the latest research on chromosomal deletions here.

Yeast as a Model Organism (Yeast Model)

Yeast is a unicellular eukaryote that is utilized and manipulated in biological research to examine biological processes that underlie DNA replication, cell growth, and division, among numerous others. This feed focuses on yeast as a model organism.

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