Mutagenic Potential ofBos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description

BioMed Research International
R P AraldiR C Stocco

Abstract

Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoprotein per se remain unknown. This study aimed to evaluate the mutagenic potential of Bos taurus papillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 μg/mL of BPV-1 E6 oncoprotein and 50 μg/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesis per se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesis per se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Resu...Continue Reading

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Citations

Jul 30, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rodrigo Pinheiro AraldiRita de Cassia Stocco
Feb 18, 2017·Genetics and Molecular Biology·Rodrigo Pinheiro AraldiWilly Beçak
Jun 1, 2021·Frontiers in Veterinary Science·Brígida Kussumoto de AlcântaraAmauri Alcindo Alfieri
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rodrigo Pinheiro AraldiEdislane Barreiros de Souza

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Methods Mentioned

BETA
PCR
electrophoresis
Assay

Software Mentioned

BioEstat

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