PMID: 7035935Oct 1, 1981Paper

Mutagenicity and cytotoxicity of haloethanes as studied in the CHO/HGPRT system

Mutation Research
E L Tan, A W Hsie

Abstract

When haloethanes were being tested as direct-acting agents in the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyl transferase (CHO/HGPRT) system, ethylene dibromide (EtBr2) exhibited more cytotoxic and mutagenic activity than ethylene dichloride (EtCl2), and the mixed halogenated congener ethylene bromochloride (EtBrCl) had an intermediate effect. On a molar basis, the relative mutagenic activity of EtBr2 : EtBrCl : EtCl2 was approximately 100 : 6 : 1. Cell survival was reduced to 50% by approx. 3, 6 and 50 mM of EtBr2, EtBrCl and EtCl2, respectively, and declined precipitously with increasing concentrations of the haloethanes. When these 3 haloethanes were assayed in the presence of S9, there was a 5-25-fold increase in cytotoxicity; however, only EtBrCl and EtCl2 also showed a concomitant increase in mutagenicity of 4-fold. The mutagenicity of EtBr2 remained unchanged when assayed in the presence of S9. When NADP was omitted from the S9 mix, which contains a NADPH-regenerating system, the increase in cytotoxicity and mutagenicity observed with the complete S9 mix was abolished. EtBr2 was shown to possess a molar equivalent mutagenic activity to ethyl methanesulfonate under the conditions of the assay. The cytot...Continue Reading

References

May 1, 1977·Journal of Toxicology and Environmental Health·U Rannug, C Ramel
Oct 1, 1977·Nature·J P O'Neill, A W Hsie
Aug 1, 1958·The Journal of Experimental Medicine·J H TJIO, T T PUCK

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Citations

Jan 1, 1990·Teratogenesis, Carcinogenesis, and Mutagenesis·A W HsieP Hrelia
Jan 5, 1996·Chemico-biological Interactions·L W WormhoudtN P Vermeulen
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hailiang HuRichard A Gatti
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·C L LimoliJ E Cleaver
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Y DaiP A Jeggo
Jan 1, 1982·Drug and Chemical Toxicology·I W DavidsonJ C Parker
Jan 25, 2011·Mutation Research·Maureen R GwinnKathryn Z Guyton
Jan 12, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·John DoullStephen Olin
Jan 1, 1983·Annals of the New York Academy of Sciences·D F Krahn
Dec 21, 2006·Journal of Occupational Health·Kasuke NaganoTaijiro Matsushima
Aug 25, 2020·Journal of Applied Toxicology : JAT·Matthew J LeBaronDarrell R Boverhof
Jan 1, 1988·Environmental and Molecular Mutagenesis·A K Giri, S S Que Hee

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