PMID: 6990231Apr 1, 1980Paper

Mutagenicity of 1-fluoro-2,4-dinitrobenzene is affected by bacterial glutathione

Mutation Research
K H Summer, W Göggelmann

Abstract

Recently we have shown that Salmonella typhimurium tester strains have high levels of the tripeptide glutathione (GSH) and activity of GSH S-transferases (Summer et al., 1979). In continuation of the GSH-dependent suppression of mutagenicity of 1-chloro-2,4-dinitrobenzene in presence of S9 fraction (Summer et al., 1979), this paper is focused on the GSH-dependent detoxifying capacity of the bacterial tester strains. 1-Fluoro-2,4-dinitrobenzene (FDNB), an electrophilic agent, which is used to identify terminal amino acids in proteins (Sanger reagent), readily reacts with GSH leading to a dose-dependent depletion of bacterial GSH. Additionally, FDNB is a strong mutagen for Salmonella typhimurium TA100, TA1538, and TA98 without metabolic activation. Presumably owing to conjugation with bacterial GHS, FDNB in concentrations which were lower or equal to those of bacterial GSH were found to be not mutagenic. Accordingly, increasing amounts of bacteria in the test system require increasing amounts of FDNB for expression of mutagenicity.

References

Jan 1, 1978·International Review of Cytology·N S Kosower, E M Kosower
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Feb 1, 1975·Mutation Research·D R McCallaP L Olive
Sep 15, 1975·European Journal of Biochemistry·H Sies, K H Summer
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Feb 1, 1974·Mutation Research·D R McCalla, D Voutsinos

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Citations

Sep 1, 1993·Molecular Microbiology·G P FergusonI R Booth

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