PMID: 7515161Jun 1, 1994Paper

Mutagenicity of tryptophan photoproducts in the Ames Salmonella assay

Mutation Research
M SjögrenU Rannug

Abstract

During the photolysis of tryptophan a large number of products is formed. In this study, aqueous solutions of tryptophan were irradiated by ultraviolet light during 5, 20 or 40 h. Each of the irradiated batches was divided into two aliquots, which were freeze-dried or extracted with chloroform. For each batch the latter extract was subsequently divided into a purified chloroform extract and a methanol extract. Aliquots of the purified chloroform extracts were fractionated and pooled, peakwise, into seven fractions. A recombined sample was also constructed. All extracts and samples were tested for mutagenicity using the standard Ames Salmonella assay. The results indicate an exposure time dependent increase in mutagenicity of the extracts, as seen with tester strain TA100 both with and without metabolic activation. The mutagenicity of the freeze-dried extracts well approximated the mutagenicity of the chloroform extracts, indicating that most mutagenicity can be extracted with chloroform. With the fractions the highest mutagenic responses were seen in the late, i.e., less lipophilic fractions. This response pattern seen in TA98 and TA100, mainly with S9 activation, was in contrast to the response of TA102 without S9, which was h...Continue Reading

References

Jul 1, 1977·Mutation Research·T MatsumotoH Okamoto
Jan 1, 1992·Environmental and Molecular Mutagenesis·U RannugH Cederberg
May 1, 1983·Mutation Research·D M Maron, B N Ames

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Citations

Jun 5, 2010·Mutagenesis·Mark D EvansMarcus S Cooke
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TateishiM Yamaizumi
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karen M VasquezPeter M Glazer
Feb 24, 1995·The Journal of Biological Chemistry·T MatsudaF Hanaoka
Dec 5, 1998·The Journal of Biological Chemistry·G DianovV A Bohr
Jun 22, 1999·The Journal of Biological Chemistry·M Wakasugi, A Sancar
Sep 12, 1997·The Journal of Biological Chemistry·S NocentiniJ M Egly
Dec 18, 2002·The Journal of Biological Chemistry·Jin-Sam YouSuk-Hee Lee
Jul 11, 2006·The Journal of Biological Chemistry·Deborah L CroteauBennett Van Houten

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