Mutanase from Paenibacillus sp. MP-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and Streptococcus mutans biofilm.

Biotechnology Letters
M PleszczyńskaJ Szczodrak

Abstract

Laetiporus sulphureus is a source of α-1,3-glucan that can substitute for the commercially-unavailable streptococcal mutan used to induce microbial mutanases. The water-insoluble fraction of its fruiting bodies from 0.15 to 0.2% (w/v) induced mutanase activity in Paenibacillus sp. MP-1 at 0.35 μ ml(-1). The mutanase extensively hydrolyzed streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h. It also degraded α-1,3-polymers of biofilms, formed in vitro by Streptococcus mutans, even after only 3 min of contact.

References

Sep 26, 2006·Journal of Dental Research·A F Paes LemeJ A Cury
Feb 21, 2007·Biotechnology Letters·M PleszczyńskaJ Szczodrak
Sep 19, 2008·Preparative Biochemistry & Biotechnology·Małgorzata PleszczyńskaJanusz Szczodrak

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Citations

Dec 12, 2012·World Journal of Microbiology & Biotechnology·Małgorzata PleszczyńskaJanusz Szczodrak
Jun 20, 2012·Preparative Biochemistry & Biotechnology·Małgorzata PleszczyńskaJanusz Szczodrak
Sep 18, 2012·Protein Expression and Purification·Małgorzata PleszczyńskaJanusz Szczodrak
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Wasana SuyothaMamoru Wakayama
Mar 13, 2016·Biotechnology and Applied Biochemistry·Małgorzata PleszczyńskaJanusz Szczodrak
Nov 26, 2019·Indian Journal of Microbiology·Sudheer Kumar BuddanaReddy Shetty Prakasham
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarzyna ZłotkoAndrzej Bieganowski

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Methods Mentioned

BETA
protein assay

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