PMID: 9654102Jul 8, 1998Paper

Mutant analysis reveals an alternative pathway for N-linked glycosylation in Drosophila melanogaster

European Journal of Biochemistry
D B RobertsP M Rudd

Abstract

The mas-1 gene of Drosophila melanogaster encodes Golgi mannosidase I (MAS-1), and flies homozygous for small deletions of the gene are viable. They show but few abnormalities and those have a low penetrance [Kerscher, S., Albert, S., Wucherpfennig, D., Heisenberg, M. & Schneuwly, S. (1995) Dev. Biol. 168, 613-626]. Here we sequence the N-linked oligosaccharides associated with a reporter protein, and with membrane proteins prepared from wild type and MAS-1 null organisms. The results show that the null organisms synthesise the same range of oligosaccharides as wild type, albeit with different ratios. There is an accumulation of the Man8GlcNAc2 which is one of the substrates for the MAS-1 enzyme. This supports the suggestion of Kerscher et al. that the lack of severe phenotypic disturbances in the null organisms is due to the presence of an alternative pathway(s), but it also shows that this alternative pathway(s) does not entirely compensate for the normal pathway.

Citations

Apr 27, 2012·Glycoconjugate Journal·Iain B H Wilson
Aug 28, 2013·Glycoconjugate Journal·Ivana NemčovičováIain B H Wilson
Sep 1, 2001·Biochimie·F AltmannI B Wilson
Apr 15, 2000·Glycobiology·Z KawarD L Jarvis
Aug 6, 2000·Glycobiology·A Seppo, M Tiemeyer
Oct 23, 2003·Annual Review of Cell and Developmental Biology·E Sergio Trombetta, Armando J Parodi
Jun 8, 1999·Annual Review of Immunology·K C GarciaI A Wilson
May 2, 2013·Medicinal Research Reviews·Matias MosqueiraRainer H A Fink
Nov 16, 2004·The Journal of Nutritional Biochemistry·Anette LandenbergerJanos Zempleni
Feb 1, 2007·The Journal of Biological Chemistry·Kazuhiro AokiMichael Tiemeyer
Oct 26, 1999·Journal of Molecular Biology·P M RuddI A Wilson

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