Mutant matrix metalloproteinase-9 reduces postoperative peritoneal adhesions in rats

International Journal of Surgery
Hussein M AttaShereen Gaber

Abstract

Postoperative peritoneal adhesions continue to be a major source of morbidity and occasional mortality. Studies have shown that matrix metalloproteinase-9 (MMP-9) levels are decreased postoperatively which may limits matrix degradation and participate in the development of peritoneal adhesions. In this proof-of-principle study, we evaluated the effect of gene therapy with catalytically inactive mutant MMP-9 on postoperative peritoneal adhesions in rats. Adenovirus encoding mutant MMP-9 (Ad-mMMP-9) or saline was instilled in the peritoneal cavity after cecal and parietal peritoneal injury in rats. Expression of mutant MMP-9 transcript was verified by sequencing. Adenovirus E4 gene expression, adhesion scores, MMP-9, tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1) and transforming growth factor-β1 (TGF-β1) expression were evaluated at sacrifice one week after treatment. Both mutant MMP-9 transcripts and adenovirus E4 gene were expressed in Ad-mMMP-9 treated adhesions. Adhesions severity decreased significantly (p = 0.036) in the Ad-mMMP-9-treated compared with saline-treated adhesions. Expression of MMP-9 mRNA and protein were elevated (p = 0.001 and p = 0.029, respectively) in the Ad-mMMP-9-treated ...Continue Reading

References

Jun 1, 1996·Surgery·L HolmdahlB Risberg
Feb 4, 1998·Journal of the American College of Surgeons·N F RayS Perry
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E RoebS Matern
Dec 29, 2000·Scandinavian Journal of Clinical and Laboratory Investigation·P FalkL Holmdahl
Dec 4, 2001·Fertility and Sterility·N CheginiUNKNOWN Peritoneal Healing and Adhesion Multiuniversity Study Group
Dec 4, 2001·Fertility and Sterility·N CheginiUNKNOWN Pritoneal Healing and Adhesion Multiuniversity Study Group
Apr 21, 2004·Obstetrical & Gynecological Survey·Dania Al-Jaroudi, Togas Tulandi
Mar 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin RoderfeldElke Roeb
Jul 29, 2006·Journal of the Society for Gynecologic Investigation·Zeynep AlpayMichael P Diamond
Jun 11, 2008·The Journal of Surgical Research·Walter J A BrokelmanMichel M P J Reijnen
Sep 10, 2011·JSLS : Journal of the Society of Laparoendoscopic Surgeons·Gregory D McClainJohn Kim
Dec 16, 2011·World Journal of Gastroenterology : WJG·Hussein M Atta
Mar 22, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Gregory ChristodoulidisKonstantinos Tepetes

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Citations

Oct 4, 2016·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Hussein M AttaHeba S Kamel
Sep 2, 2020·Acta Biomaterialia·Jingyi TangShengfu Chen
Jun 1, 2021·Frontiers in Immunology·Simone N ZwickyJoel Zindel
Aug 28, 2021·Biomedicines·Ali Fatehi HassanabadPaul W M Fedak

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