Mutant prevention concentrations for single-step fluoroquinolone-resistant mutants of wild-type, efflux-positive, or ParC or GyrA mutation-containing Streptococcus pneumoniae isolates

Antimicrobial Agents and Chemotherapy
Heather SmithDaryl J Hoban

Abstract

Three fluoroquinolone-susceptible and five fluoroquinolone-resistant (two with ParC Ser79Phe mutations, one with a GyrA Ser81Phe mutation, and two that were efflux positive) Streptococcus pneumoniae isolates were exposed to one, two, four, eight, and sixteen times the MICs of ciprofloxacin, gatifloxacin, gemifloxacin, levofloxacin, and moxifloxacin. Mutational frequencies were calculated at each multiple of the MIC for which growth was observed. Mutant prevention concentrations (MPCs) and the multiple of the MIC at the MPC (MP(MIC)) were evaluated. All resulting mutants were sequenced for quinolone resistance-determining region changes in GyrA and ParC and were evaluated for reserpine-sensitive efflux. The MPC order was generally ciprofloxacin > levofloxacin > gatifloxacin > moxifloxacin > gemifloxacin. The MP(MIC) order varied depending on the genetic constitution of the original isolates from which the mutants were generated. For those mutants created from fluoroquinolone-susceptible isolates (those that had wild-type ParC and GyrA and were efflux negative), the MP(MIC) order was ciprofloxacin = moxifloxacin > gemifloxacin > levofloxacin > gatifloxacin. The MP(MIC)s of each fluoroquinolone for mutants created from isolates wi...Continue Reading

References

Oct 1, 1996·Antimicrobial Agents and Chemotherapy·X S PanL M Fisher
Oct 9, 1998·The Journal of Biological Chemistry·A B Khodursky, N R Cozzarelli
Aug 18, 1999·The American Journal of Medicine·P Ball
Dec 10, 1999·The Journal of Antimicrobial Chemotherapy·J M Legg, A J Bint
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·G SindelarK Drlica
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M PutmanW N Konings
Feb 13, 2001·Antimicrobial Agents and Chemotherapy·J M BlondeauK Drlica
Mar 16, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D C Hooper
Aug 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·X Zhao, K Drlica
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Angela B BrueggemannGary V Doern
Mar 8, 2002·The New England Journal of Medicine·Ross DavidsonDonald E Low
Aug 2, 2003·Emerging Infectious Diseases·Mina Ebrahimi-RadBrigitte Gicquel

❮ Previous
Next ❯

Citations

Jun 9, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George A Jacoby
Aug 19, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sara K Olofsson, Otto Cars
Mar 24, 2010·Antimicrobial Agents and Chemotherapy·Kim CreditoPeter C Appelbaum
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Heather J Smith-AdamGeorge G Zhanel
Aug 30, 2008·Journal of Cataract and Refractive Surgery·Stephen S LaneShaleen Belani
Aug 19, 2007·Archivos de bronconeumología·Salvador Bello Dronda, Manuel Vilá Justribó
Nov 11, 2006·Médecine et maladies infectieuses·P Chavanet, D Croisier
May 10, 2013·Current Eye Research·Bianka SobolewskaEfdal Yoeruek
Jan 19, 2008·Expert Opinion on Pharmacotherapy·Javier AspaFelipe Rodríguez de Castro
Mar 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F Van BambekeP M Tulkens
Dec 5, 2006·Expert Review of Anti-infective Therapy·Joseph P LynchGeorge G Zhanel
Dec 6, 2019·MedChemComm·Thu D M PhamMark A T Blaskovich

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.