Mutant rat strain lacking D-amino-acid oxidase

Amino Acids
R KonnoA Niwa

Abstract

D-amino-acid oxidase (DAO) is known to be associated with schizophrenia. Since the expression of DAO gene had been reported to be very low in LEA rats, we examined LEA/SENDAI rats in detail. These rats did not have DAO activity, enzyme protein or mRNA encoding this enzyme. Sequencing of the 5'-upstream region of the DAO gene revealed the deletion of one triplet in the 15 TAA repeats approximately 700-bp upstream of the transcription start point. A 1.3-kb upstream fragment containing the TAA repeats and the transcription start point was inserted into a reporter vector and was transfected into COS-1, NRK-52E and CCL-PK1 cells. Although the fragments containing 15 or 14 repeats had high promoter activity, the fragment containing 13 repeats had very weak activity. Electrophoretic mobility-shift assays showed that the nuclear extracts from COS-1 and COS-7 cells had proteins that bound to the oligonucleotides containing the TAA repeats. These results suggest that the TAA repeats are important for expression of the DAO gene. The LEA/SENDAI rats lacking DAO would be a useful tool for the investigations aimed at the elucidation of the relationships between this flavoenzyme and schizophrenia.

References

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Citations

May 22, 2013·Journal of Diabetes Research·Tadashi OkamuraNoriyuki Kasai
Aug 16, 2012·Amino Acids·Masahiro YamanakaRyuichi Konno
Jul 16, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hiroko OhideRyuichi Konno
Sep 21, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yurika MiyoshiKiyoshi Zaitsu
Jun 22, 2010·Chemistry & Biodiversity·Ryuichi KonnoKiyoshi Zaitsu
Jul 6, 2016·Frontiers in Microbiology·Yingchao LiTingyue Gu
Jun 16, 2012·Toxicologic Pathology·Zaher A RadiWalter F Bobrowski
Jul 15, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tsutomu SasakiTadahiro Kitamura
Dec 29, 2018·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Yukiko ShimizuTadashi Okamura
Jun 9, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Yukiko ShimizuTadashi Okamura

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