Mutant rho factors with increased transcription termination activities. II. Identification and functional dissection of amino acid changes.

Journal of Molecular Biology
H MoriK Shigesada

Abstract

We have determined the nucleotide sequences of three mutant rho genes encoding hyperfunctional rho proteins (rho S) together with their parent allele, rho-ts702. These mutant rho factors contain the following amino acid changes as deduced from their sequences: (1) the thermo-labile mutant, rho-ts702, has Thr304 substituting for Ala; (2) rho S-77 and rho S-81, which are selectively altered in the primary polynucleotide binding site, share an identical mutation, Leu3----Phe; (3) rho S-82, which is altered in both the primary and secondary polynucleotide binding sites, carries three amino acid substitutions together, Leu3----Phe, Asp156----Asn and Thr323----Ile. Dissection and functional characterization of each mutation in rho S-82 have revealed that Ile323 alone is responsible for alterations in both the secondary RNA interaction and the terminator selectivity observed with the original mutant, rho S-82. Taken together, these results not only confirm our proposal in the accompanying paper that the primary and secondary RNA binding sites differently contribute in determining the overall efficiency and site-specificity of termination, respectively, but also support the possibility that these binding sites exist as structurally dis...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A DasS Adhya
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H InokoM Imai
Nov 6, 1987·Cell·M P Wickens, J E Dahlberg
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A J Dombroski, T Platt
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D C FryA S Mildvan
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Dec 20, 1969·Nature·J W Roberts
Apr 15, 1980·Journal of Molecular Biology·G R Galluppi, J P Richardson
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y OhshimaT Miyata
Dec 25, 1982·Journal of Molecular Biology·F SangerG B Petersen
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jun 11, 1983·Nucleic Acids Research·J L Pinkham, T Platt
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 17, 1984·Biochemistry·G D Brayer, A McPherson
Jan 31, 1984·Biochemistry·R V PrigodichJ E Coleman
May 15, 1981·Journal of Molecular Biology·J BrosiusH F Noller
Mar 25, 1982·Journal of Molecular Biology·L R Finger, J P Richardson

❮ Previous
Next ❯

Citations

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J JinC A Gross
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alejandra Rodríguez-VerdugoAlbert F Bennett
Aug 31, 2000·Annual Review of Biochemistry·S S Patel, K M Picha
Mar 30, 2001·The Journal of Biological Chemistry·D E Kim, S S Patel
Nov 7, 1999·The Journal of Biological Chemistry·D E Kim, S S Patel
Jan 19, 1996·The Journal of Biological Chemistry·J P Richardson
Jul 22, 2017·Annual Review of Microbiology·Pallabi MitraRanjan Sen
Feb 19, 2004·The Journal of Biological Chemistry·Yong-Joo JeongSmita S Patel
Apr 17, 1999·The Journal of Biological Chemistry·D E KimS S Patel
May 9, 2021·Applied Microbiology and Biotechnology·Tomás G VillaAngeles Sánchez-Pérez
Apr 6, 1990·Biochimica Et Biophysica Acta·J P Richardson
Aug 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Beate SchwerStewart Shuman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.