PMID: 10275Oct 1, 1976

Mutant strains (nit) of Salmonella typhimurium with a pleiotropic defect in nitrogen metabolism

Journal of Bacteriology
J BroachS Kustu

Abstract

We have isolated mutant strains (nit) of Salmonella typhimurium that are defective in nitrogen metabolism. They have a reduced ability to use a variety of compounds including glutamate, proline, arginine, N-acetyl-glucosamine, alanine, and adenosine as sole nitrogen source. In addition, although they grow normally on high concentrations of ammonium chloride (greater than 1 mM) as nitrogen source, they grow substantially more slowly than wild type at low concentrations (less than 1 mM). We postulated that the inability of these strains to utilize low concentrations of ammonium chloride accounts for their poor growth on other nitrogen sources. The specific biochemical lesion in strains with a nit mutation is not known; however, mutant strains have no detectable alteration in the activities of glutamine synthetase, glutamate synthetase, or glutamate dehydrogenase, the enzymes known to be involved in assimilation of ammonia. A nit mutation is suppressed by second-site mutations in the structural gene for glutamine synthetase (glnA) that decrease glutamine synthetase activity.

Citations

May 6, 2003·Annual Review of Microbiology·Larry Reitzer
Sep 1, 1990·Molecular & General Genetics : MGG·R B Helling
Nov 23, 2006·Physiology·Shahram Khademi, Robert M Stroud
Jul 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jason A Hall, Sydney Kustu
Mar 1, 1989·Antonie van Leeuwenhoek·J H Klemme
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Franz GruswitzRobert M Stroud
Jun 22, 2012·PloS One·Jessica De IngeniisLeonardo Sorci
Dec 4, 2013·Microbiology and Molecular Biology Reviews : MMBR·Wally C van HeeswijkFred C Boogerd
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dalai Yan

Related Concepts

Acetylglucosamine
Oxidoreductases Acting on CH-NH Group Donors
Ammonium Chloride
Genome Mapping
Chromosomes, Bacterial
Cistron
Glutamate Dehydrogenase
Glutamate Synthase
Glutamic Acids
Glutamate-Ammonia Ligase

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