Mutant T cell lines as model systems for the dissection of T cell antigen receptor signaling pathways

Immunologic Research
R T Abraham

Abstract

T cell antigen receptor (TCR) ligation triggers a cascade of intracellular signaling events that culminate in T cell activation, cytokine gene expression, differentiation, or apoptosis. Many of the enzymes and adapter proteins responsible for signal propagation from the cell surface TCR to the cytoplasm and nucleus have now been identified and molecularly cloned. However, a comprehensive understanding of the regulation and functions of these signaling proteins in T cells remains a major challenge. Our laboratory has approached this problem through the generation of a panel of Jurkat T cell-derived somatic mutants that fail to express several critical elements in the TCR-linked signaling cascade. This review highlights the use of mutant T cell lines for functional characterizations of two of these signaling proteins--the ZAP-70 tyrosine kinase and phospholipase C-gamma1.

Citations

Dec 7, 2011·The Journal of Biological Chemistry·Chanyu YueAna M Gamero
Sep 18, 2002·The EMBO Journal·Youjia CaoRobert T Abraham
Apr 3, 2003·Immunological Reviews·Maria-Cristina Seminario, Ronald L Wange
Apr 7, 2011·European Journal of Immunology·Gregory F WuJonathan S Maltzman
Feb 1, 2006·Immunobiology·Leandro J CarreñoAlexis M Kalergis
Dec 22, 2005·Molecular & Cellular Proteomics : MCP·Imran H KhanPaul A Luciw
Apr 2, 2004·Nature Reviews. Immunology·Robert T Abraham, Arthur Weiss
Jul 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rong ZengJanis K Burkhardt
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek DombroskiPamela L Schwartzberg
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek D SloanKeith R Jerome
Jun 7, 2003·Advances in Enzyme Regulation·Ezio BonviniBarbara L Rellahan

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