Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin.

Infection and Immunity
L de HaanJ Wilschut

Abstract

The Escherichia coli heat-labile enterotoxin (LT) is a potent inducer of mucosal immune responses. In a previous study (L. DeHaan, W. R. Verweij, M. Holtrop, E. Agsteribbe, and J. Wilschut, Vaccine 14:620-626, 1996), we have shown that efficient induction of an LTB-specific mucosal immune response by LT requires the presence of the LTA chain, suggesting a possible role of the enzymatic activity of LTA in the induction of these responses. In the present study, we generated LT mutants with altered ADP-ribosylation activities and evaluated their immunogenicity upon intranasal administration to mice. The results demonstrate that the mucosal immunogenicity of LT is not dependent on its ADP-ribosylation activity.

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Citations

Sep 3, 2009·Expert Review of Vaccines·Kosuke Kataoka, Kohtaro Fujihashi
Mar 17, 2001·Scandinavian Journal of Immunology·M YamamotoH Kiyono
Nov 15, 2001·Animal Health Research Reviews·D L Foss, M P Murtaugh
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Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yukari HagiwaraKohtaro Fujihashi
Sep 3, 2004·Nature Reviews. Immunology·Hiroshi Kiyono, Satoshi Fukuyama
Jan 7, 1998·Protein Science : a Publication of the Protein Society·F van den AkkerW G Hol
Jun 20, 2002·Veterinary Immunology and Immunopathology·Michael P Murtaugh, Dennis L Foss
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May 20, 1998·Research in Immunology·N Lycke
Oct 3, 1999·Vaccine·G Del Giudice, R Rappuoli

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