PMID: 3753980Mar 1, 1986Paper

Mutants of the membrane-binding region of Semliki Forest virus E2 protein. I. Cell surface transport and fusogenic activity

The Journal of Cell Biology
D F Cutler, H Garoff

Abstract

Three mutations of the membrane-binding region of the Semliki Forest virus (SFV) p62 polypeptide (the precursor for virion E3 and E2) have been made by oligonucleotide-directed mutagenesis of a cDNA clone encoding the SFV structural proteins. One of the mutations (A2) substitutes a Glu for an Ala in the middle of the hydrophobic stretch which spans the bilayer. A1 and A3 alter the two basic charged amino acids in the cytoplasmic domain next to the hydrophobic region. The wild-type charge cluster of Arg-Ser-Lys (+2) has been changed to Gly-Ser-Met (0;A3) or to Gly-Ser-Glu (-1;A1). The mutant p62 proteins have been analyzed both in the presence and the absence of E1, the other half of the heterodimer spike complex of SFV. The mutant proteins expressed in COS-7 cells are glycosylated and are of the expected sizes. When co-expressed with E1, all three mutants are cleaved to yield the E2 protein and transported to the surface of COS-7 cells. When expressed in the absence of E1, the mutant p62 proteins remain uncleaved but still reach the cell surface. Once at the cell surface, all three mutants, when co-expressed with E1, can promote low pH-triggered cell-cell fusion. These results show that the three mutant p62/E2 proteins are stil...Continue Reading

References

Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R HenningG M Edelman
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F N KatzH F Lodish
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J MessingP Hans Hopschneider
Jan 5, 1985·Journal of Molecular Biology·N G DavisP Model
Dec 1, 1970·Pielȩgniarka i połozna·M Wiśniewski
Dec 21, 1984·Nucleic Acids Research·W KramerH J Fritz
Jan 1, 1984·Biology of the Cell·M Hortsch, D I Meyer
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J D Boeke, P Model
Mar 25, 1983·Nucleic Acids Research·L DenteR Cortese
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
May 1, 1983·Quarterly Reviews of Biophysics·J WhiteA Helenius
Aug 1, 1983·Cell·M KressG Jay
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik
Nov 25, 1981·Journal of Molecular Biology·G P Dotto, K Horiuchi
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
Next ❯

Citations

May 1, 1992·The Journal of Cell Biology·T Marquardt, A Helenius
Feb 1, 1995·The Journal of Cell Biology·B U BarthH Garoff
Jul 25, 2014·PLoS Neglected Tropical Diseases·James Weger-LucarelliJorge E Osorio
Dec 1, 1989·Molecular and Cellular Biology·H HomaredaH Matsui
Sep 1, 1994·Microbiological Reviews·J H Strauss, E G Strauss
Aug 1, 1991·Journal of Virology·P Levy-Mintz, M Kielian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.