Mutants of the mini-F plasmid pML31 thermosensitive in replication.

Journal of Bacteriology
R Eichenlaub

Abstract

Hydroxylamine mutagenesis was used for the induction of thermosensitive replication mutants of the mini-F plasmid pML31. Replication mutants were characterized by studying the segregation kinetics and the incorporation of [3H]-thymidine into plasmid deoxyribonucleic acid at the nonpermissive temperature. Based on these experiments two types of mutants could be distinguished. Mutants of type I are fast segregating with the kinetics expected if plasmid replication was blocked immediately. Double-label experiments showed a rapid shut-off of replication in these mutants at 42 degrees C. Mutants of type II segregate slower, showing only a partial inhibition of plasmid deoxyribonucleic acid synthesis at the nonpermissive temperature. The label incorporated at 42 degrees C was predominantly found in open circular plasmid molecules.

References

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·S MakiT Horiuchi
Jan 1, 1980·Molecular & General Genetics : MGG·H Wehlmann, R Eichenlaub
Jan 1, 1980·Molecular & General Genetics : MGG·R Eichelaub, H Wehlmann
Jan 1, 1985·Molecular & General Genetics : MGG·S SommerR Devoret
May 1, 1993·Molecular & General Genetics : MGG·S SommerR Devoret
Sep 1, 1981·Journal of Bacteriology·J Ebbers, R Eichenlaub
Dec 1, 1989·Journal of Bacteriology·F TurnowskyG Högenauer
Mar 1, 1984·Microbiological Reviews·J R Scott
Nov 5, 1984·Journal of Molecular Biology·A BrandenburgerR Devoret
Nov 8, 1991·Science·E S HaasN R Pace

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