Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii.

Genetics
S W JamesP A Lefebvre

Abstract

We have used genetic analysis to study the mode of action of two anti-microtubule herbicides, amiprophos-methyl (APM) and oryzalin (ORY). Over 200 resistant mutants were selected by growth on APM- or ORY-containing plates. The 21 independently isolated mutants examined in this study are 3- to 8-fold resistant to APM and are strongly cross-resistant to ORY and butamiphos, a close analog of APM. Two Mendelian genes, apm1 and apm2, are defined by linkage and complementation analysis. There are 20 alleles of apm1 and one temperature-sensitive lethal (33 degrees) allele of apm2. Mapping by two-factor crosses places apm1 6.5 cM centromere proximal to uni1 and within 4 cM of pf7 on the uni linkage group, a genetically circular linkage group comprising genes which affect flagellar assembly or function; apm2 maps near the centromere of linkage group VIII. Allele-specific synthetic lethality is observed in crosses between apm2 and alleles of apm1. Also, self crosses of apm2 are zygotic lethal, whereas crosses of nine apm1 alleles inter se result in normal germination and tetrad viability. The mutants are recessive to their wild-type alleles but doubly heterozygous diploids (apm1 +/+ apm2) made with apm2 and any of 15 apm1 alleles display...Continue Reading

Citations

Sep 24, 2011·Genetics·Carolyn D SilflowPaul A Lefebvre
Aug 19, 1999·Biosensors & Bioelectronics·D SchubnellC F Beck
Oct 6, 1989·Cell·U W Goodenough
Jan 1, 1989·Cell Motility and the Cytoskeleton·S K Dutcher, F G Lux
Jan 1, 1989·Cell Motility and the Cytoskeleton·M T FullerT S Hays
Jan 27, 2004·Molecular Biology of the Cell·Naomi S MorrissetteL David Sibley
Jan 1, 1991·Pharmacology & Therapeutics·L C Morejohn, D E Fosket

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